bs-2762R [Primary Antibody]
DARPP32 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DARPP32

Immunogen Range: 51-150/204


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 84152

Swiss Prot: Q9UD71

Source: KLH conjugated synthetic peptide derived from human DARPP32

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background:

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 23


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Horse
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: mouse lung lysates Lane 2: mouse intestine lysates probed with Anti DARPP32 Polyclonal Antibody, Unconjugated (bs-2762R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 23kD. Observed band size: 23kD.


Formalin-fixed and paraffin embedded rat brain labeled with Anti DARPP32 Polyclonal Antibody, Unconjugated (bs-2762R) at 1:200 followed by conjugation to the secondary antibody and DAB staining