bs-2771R [Primary Antibody]
AMPK alpha 2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AMPK alpha 2

Immunogen Range: 351-450/552


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5563

Swiss Prot: P54646

Source: KLH conjugated synthetic peptide derived from human AMPK alpha 2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 62


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Min, et al. "Metformin ameliorates hepatic steatosis and improves the induction of autophagy in HFD‑induced obese mice." Molecular Medicine Reports.Read more>>
  • Shi C et al. Acetaminophen aggravates fat accumulation in NAFLD by inhibiting autophagy via the AMPK/mTOR pathway. European Journal of Pharmacology.2019.Read more>>
  • Zhang Q et al. CTRP13 attenuates the expression of LN and CAV-1 Induced by high glucose via CaMKK/AMPK pathway in rLSECs. Aging (Albany NY). 2020 Jun 17;12(12):11485-11499.Read more>>
  • Liu J et al. Obesity Potentiates Esophageal Squamous Cell Carcinoma Growth and Invasion by AMPK-YAP PathwayJ Immunol Res.2020 Dec 10;2020:6765474.Read more>>
  • Songsong Jiang. et al. A Comparison Study on the Therapeutic Effect of High Protein Diets Based on Pork Protein versus Soybean Protein on Obese Mice. FOODS. 2022 Jan;11(9):1227Read more>>
  • Wei Ruyuan. et al. Silencing TUFM Inhibits Development of Monocrotaline-Induced Pulmonary Hypertension by Regulating Mitochondrial Autophagy via AMPK/mTOR Signal Pathway. OXID MED CELL LONGEV. 2022;2022:4931611Read more>>
  • Shuang-Feng Xu. et al. Astrocyte-specific loss of lactoferrin influences neuronal structure and function by interfering with cholesterol synthesis. GLIA. 2022 AugRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human kidney labeled with Anti-PRKAA2/AMPK alpha 2 Polyclonal Antibody, Unconjugated (bs-2771R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti PRKAA2/AMPK alpha 2 Polyclonal Antibody, Unconjugated (bs-2771R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Antigen: bs-2771P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450


Lane 1: Human Hela cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human 293T cell lysates probed with AMPK alpha 2 Polyclonal Antibody, Unconjugated (bs-2771R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.