bs-2773R [Primary Antibody]
TNFRSF13B Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TNFRSF13B

Immunogen Range: 21-120/249


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 57916

Swiss Prot: Q9ET35

Source: KLH conjugated synthetic peptide derived from mouse TNFRSF13B

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T-cell function and the regulation of humoral immunity (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 32


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

L1 mouse brain lysates L2 mouse heart lysates probed with Anti TNFRSF13B Polyclonal Antibody, Unconjugated (bs-2773R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 32kD. Observed band size: 32kD


Lane 1: Mouse Spleen lysates; Lane 2: Mouse Lymph node lysates probed with TNFRSF13B Polyclonal Antibody, Unconjugated (bs-2773R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Spleen lysates; Lane 2: Mouse Lymph node lysates; Lane 3: Mouse Thymus lysates; Lane 4: Mouse Bone lysates probed with TNFRSF13B Polyclonal Antibody, Unconjugated (bs-2773R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Mouse Lung tissue lysates; Lane 5: Rat Stomach tissue lysates probed with TNFRSF13B Polyclonal Antibody, Unconjugated (bs-2773R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.