bs-2779R [Primary Antibody]
CD44v7 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD44v7

Specificity: This antibody will detect isoform 7 exclusively.

Immunogen Range: 201-300/340


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 960

Swiss Prot: P16070

Source: KLH conjugated synthetic peptide derived from human CD44v7

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 37


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human endometrial cancer labeled with Anti-CD44v7 Polyclonal Antibody, Unconjugated (bs-2779R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Human Hela cell lysates; Lane 2: Human PANC-1 cell lysates; Lane 3: Human HUVEC cell lysates; Lane 4: Human A549 cell lysates probed with CD44v7 Polyclonal Antibody, Unconjugated (bs-2779R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.