DATASHEET
Host:
Rabbit
Target Protein:
SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T221)
Modification Site:
SIK1+ SIK2+ SIK3
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
150094
Swiss Prot:
P57059
Source:
KLH conjugated synthetic peptide derived from human SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T221)
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Serine/threonine-protein kinase involved in variousprocesses such as cell cycle regulation, gluconeogenesis andlipogenesis regulation, muscle growth and differentiation and tumorsuppression. Phosphorylates HDAC4, HDAC5, PPME1, SREBF1,TORC1/CRTC1 and TORC2/CRTC2. Acts as a tumor suppressor and plays akey role in p53/TP53-dependent anoikis, a type of apoptosistriggered by cell detachment: required for phosphorylation ofp53/TP53 in response to loss of adhesion and is able to suppressmetastasis. Part of a sodium-sensing signaling network, probably bymediating phosphorylation of PPME1: following increases inintracellular sodium, SIK1 is activated by CaMK1 and phosphorylatesPPME1 subunit of protein phosphatase 2A (PP2A), leading todephosphorylation of sodium/potassium-transporting ATPase ATP1A1and subsequent increase activity of ATP1A1. Acts as a regulator ofmuscle cells by phosphorylating and inhibiting class II histonedeacetylases HDAC4 and HDAC5, leading to promote expression of MEF2target genes in myocytes. Also required during cardiomyogenesis byregulating the exit of cardiomyoblasts from the cell cycle viadown-regulation of CDKN1C/p57Kip2. Acts as a regulator of hepaticgluconeogenesis by phosphorylating and repressing the CREB-specificcoactivators TORC1/CRTC1 and TORC2/CRTC2, leading to inhibit CREBactivity. Also regulates hepatic lipogenesis by phosphorylating andinhibiting SREBF1.
Conjugation:
ALEXA FLUOR® 594
Excitation/ Emission:
590nm/617nm
Size:
100ul
Concentration:
1ug/ul
Applications:
WB(1:300-5000)
49
Human
Predicted Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.