DATASHEET
Host:
Rabbit
Target Protein:
V-set and transmembrane domain-containing protein 1
Immunogen Range:
111-209/209
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
100360054
Source:
KLH conjugated synthetic peptide derived from rat VSTM1
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Isoform 2: Behaves as a cytokine, promoting IL17A secretion by CD4+ T-cells, and differentiation and activation of IL17 producing helper T-cells (TH17). Isoform 1: Inhibitory immune receptor involved in the regulation of phagocytes.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VSTM1) Polyclonal Antibody, Unconjugated (bs-2861R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Rat Bone tissue lysates; Lane 2: Rat Spleen tissue lysates; Lane 3: Rat Lymph tissue lysates probed with VSTM1 Polyclonal Antibody, Unconjugated (bs-2861R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.