bs-2861R [Primary Antibody]
VSTM1
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
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DATASHEET

Host: Rabbit

Target Protein: V-set and transmembrane domain-containing protein 1

Immunogen Range: 111-209/209


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 100360054

Source: KLH conjugated synthetic peptide derived from rat VSTM1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Isoform 2: Behaves as a cytokine, promoting IL17A secretion by CD4+ T-cells, and differentiation and activation of IL17 producing helper T-cells (TH17). Isoform 1: Inhibitory immune receptor involved in the regulation of phagocytes.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Predicted Molecular Weight: 24


Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VSTM1) Polyclonal Antibody, Unconjugated (bs-2861R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Lane 1: Rat Bone tissue lysates; Lane 2: Rat Spleen tissue lysates; Lane 3: Rat Lymph tissue lysates probed with VSTM1 Polyclonal Antibody, Unconjugated (bs-2861R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.