bs-2880R [Primary Antibody]
LPAR1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LPAR1

Immunogen Range: 281-364/364


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1902

Swiss Prot: Q92633

Source: KLH conjugated synthetic peptide derived from human EDG2/LPAR1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 41


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Linmu, et al. "Lysophosphatidic acid individually induces macrophage-derived foam cell formation by blocking the expression of SRBI." Biochemical and Biophysical Research Communications (2017).Read more>>
  • Kim B et al. Targeted lipid nanoemulsions encapsulating epigenetic drugs exhibit selective cytotoxicity on CDH1-/FOXM1+ triple negative breast cancer cells.Mol Pharm. 2019 May 6;16(5):1813-1826. Read more>>
  • Chen L et al. Lysophosphatidic acid decreased macrophage foam cell migration correlated with downregulation of fucosyltransferase 8 via HNF1α. Atherosclerosis. 2019 Sep 11;290:19-30. Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human pancreatic cancer labeled with Anti-EDG2/LPA1 Polyclonal Antibody, Unconjugated (bs-2880R) at 1:400 followed by conjugation to the secondary antibody and DAB staining


L1 Mouse liver lysates, L2 Mouse cerebrum lysates probed with Anti- EDG2/LPA1 Polyclonal Antibody, Unconjugated (bs-2880R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:2000 90min in RT. Predicted band and observed band size: 41kD


Lane 1: Mouse Heart lysates probed with LPAR1 Polyclonal Antibody, Unconjugated (bs-2880R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Kidney tissue lysates; Lane 2: Rat Kidney tissue lysates probed with LPA1/LPAR1 Polyclonal Antibody, Unconjugated (bs-2880R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.