bs-2922R [Primary Antibody]
AHSG Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AHSG

Immunogen Range: 201-300/367


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 197

Swiss Prot: P02765

Source: KLH conjugated synthetic peptide derived from human Alpha-2-HS-glycoprotein chain A.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 31/40


Cross Reactive Species: Mouse
Others

Predicted Cross Reactive Species: Human
Rat
Dog
Cow
Sheep
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Naito, Chisato, et al. "Facilitatory effects of fetuin-A on atherosclerosis."Atherosclerosis (2016).Read more>>
  • Wu CY et al. Ectopic calcification and formation of mineralo-organic particles in arteries of diabetic subjects. Sci Rep. 2020 May 22;10(1):8545.Read more>>
  • Yamanishi Kyosuke. et al. Exploring Molecular Mechanisms Involved in the Development of the Depression-Like Phenotype in Interleukin-18-Deficient Mice. Biomed Res Int. 2021;2021:9975865Read more>>
VALIDATION IMAGES

Lane 1: mouse liver lysates Lane 2: mouse intestine lysates probed with Anti AHSG/Fetuin A/Alpha 2 HS Glycoprotein Polyclonal Antibody, Unconjugated (bs-2922R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 31kD. Observed band size: 31kD.


Antigen: bs-2922P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450


Lane 1: Mouse Liver lysates probed with AHSG Polyclonal Antibody, Unconjugated (bs-2922R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.