bs-2925R [Primary Antibody]
Klotho Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Klotho

Immunogen Range: 401-500/1012


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9365

Swiss Prot: Q9UEF7

Source: KLH conjugated synthetic peptide derived from human Klotho

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity). The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

Size: 100ul

Concentration: 1ug/ul

Applications: IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)

Predicted Molecular Weight: 113


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Prud'homme, Gérald J., et al. "The anti-aging protein Klotho is induced by GABA therapy and exerts protective and stimulatory effects on pancreatic beta cells." Biochemical and Biophysical Research Communications (2017)Read more>>
  • Ni et al. Adipose-Derived Mesenchymal Stem Cells Transplantation Alleviates Renal Injury in Streptozotocin-Induced Diabetic Nephropathy. (2015) J.Histochem.Cytochem. 63:842-53Read more>>
  • Stenhouse Claire. et al. Novel Mineral Regulatory Pathways in Ovine Pregnancy: I. Phosphate, Klotho Signaling, and Sodium Dependent Phosphate Transporters. Biol Reprod. 2021 FebRead more>>
  • Xiao-Tan Zhang. et al. Baicalin reversal of DNA hypermethylation-associated Klotho suppression ameliorates renal injury in type 1 diabetic mouse model. Cell Cycle. 2020;19(23):3329-3347Read more>>
  • Nianbin Song. et al. Multiple genetic programs contribute to CD4 T cell memory differentiation and longevity by maintaining T cell quiescence. Cell Immunol. 2020 Nov;357:10421Read more>>
  • Amelia K. Fotheringham. et al. Kidney disease risk factors do not explain impacts of low dietary protein on kidney function and structure. Iscience. 2021 Oct;:103308Read more>>
  • Stenhouse Claire. et al. Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling. Biol Reprod. 2022 FebRead more>>
  • Li Lei. et al. LncRNA 152 attenuates lipopolysaccharide-induced acute kidney injury in rats by regulating the FGF23/Klotho/MAPK axis. NEPHROLOGY. 2023 SepRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining


Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, DAB staining.