Host: Rabbit
Target Protein: TNFRSF5
Immunogen Range: 201-277/277
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 958
Swiss Prot: P25942
Source: KLH conjugated synthetic peptide derived from human TNFRSF5
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Storage Condition: Store at -20°C for 12 months..
Background: Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-1000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Cross Reactive Species:
Human
Mouse
Rabbit
Predicted Cross Reactive Species:
Rat
Dog
Cow
Pig
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti-TNFRSF5 Polyclonal Antibody, Unconjugated (bs-2929R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
HUVEC cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TNFRSF5 Antibody（bs-2929R-FITC） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).