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Host: Rabbit
Target Protein: CD40/TNFRSF5
Immunogen Range: 201-277/277
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 958
Swiss Prot: P25942
Source: KLH conjugated synthetic peptide derived from human TNFRSF5
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100uL, 20uL
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 30
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Predicted Cross Reactive Species:
Dog
Cow
Pig
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Dong Ming. et al. FBS-Derived Exosomes as a Natural Nano-Scale Carrier for Icariin Promote Osteoblast Proliferation. Front Bioeng Biotech. 2021 Feb;9:146Read more>>
- Chengxiao Wang. et al. Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation. Carbohyd Polym. 2021 May;:118211Read more>>
- Dahan Yang. et al. Tandem Mass Tag-Based Quantitative Proteomic Analysis of Chicken Bursa of Fabricius Infected With Reticuloendotheliosis Virus. Front Vet Sci. 2021; 8: 666512Read more>>
- Xinxin Yu. et al. Nanotherapy for bone repair: milk-derived small extracellular vesicles delivery of icariin. DRUG DELIV. 2023;30(1):Article: 2169414Read more>>
Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti-TNFRSF5 Polyclonal Antibody, Unconjugated (bs-2929R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
The Raji (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) .Cells stained with Primary Antibody for 30 min at room temperature, followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD40 antibody (bs-2929R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (CD40) polyclonal Antibody, unconjugated (bs-2929R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.