bs-2932R [Primary Antibody]
Clathrin heavy chain Polyclonal Antibody
www.biossusa.com
[email protected]
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DATASHEET

Host: Rabbit

Target Protein: Clathrin heavy chain

Immunogen Range: 401-500/1675


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1213, 8218

Source: KLH conjugated synthetic peptide derived from human Clathrin heavy chain.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 192


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Mörke, Caroline. "Caveolae-mediated phagocytosis of biomaterial surface structures by human osteoblasts."Read more>>
VALIDATION IMAGES

Mouse olfactory bulb lysates probed with Clathrin heavy chain Polyclonal Antibody, Unconjugated (bs-2932R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human U-2 OS cell lysates probed with Clathrin heavy chain Polyclonal Antibody, Unconjugated (bs-2932R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.