bs-2934R [Primary Antibody]
Complement C3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Complement C3

Immunogen Range: 1551-1663/1663


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 718

Swiss Prot: P01024

Source: KLH conjugated synthetic peptide derived from human Complement C3 alpha chain

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:837664, PubMed:29953, PubMed:959512, PubMed:1432298, PubMed:15833747, PubMed:16333141, PubMed:1961575).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(IHC-P)(1:100-500)

Predicted Molecular Weight: 181


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Horse
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Trang, N. T., et al. "Membranoproliferative Glomerulonephritis in a Calf with Nephrotic Syndrome." Journal of Comparative Pathology (2014).Read more>>
  • Zhuang, Haoyang, et al. "A novel mechanism for generating the interferon signature in lupus: opsonization of dead cells by complement and IgM."Arthritis & Rheumatology (2016).Read more>>
  • Rogińska, Dorota, et al. "Depletion of the Third Complement Component Ameliorates Age-Dependent Oxidative Stress and Positively Modulates Autophagic Activity in Aged Retinas in a Mouse Model." Oxidative Medicine and Cellular Longevity 2017 (2017).Read more>>
  • Rogińska et al. Depletion of the Third Complement Component Ameliorates Age-Dependent Oxidative Stress and Positively Modulates Autophagic Activity in Aged Retinas in a Mouse Model. (2017) Oxid.Med.Cell.Longe. 2017:5306790Read more>>
  • Liao et al. Tacrolimus Protects Podocytes from Injury in Lupus Nephritis Partly by Stabilizing the Cytoskeleton and Inhibiting Podocyte Apoptosis. (2015) PLoS.One. 10:e0132724Read more>>
  • Liu B et al. Zhen-wu-tang ameliorates membranous nephropathy rats through inhibiting NF-κB pathway and NLRP3 inflammasome. Phytomedicine. 2019 Apr 2;59:152913. Read more>>
  • Bai L et al. Immunosuppressive effect of artemisinin and hydroxychloroquine combination therapy on IgA nephropathy via regulating the differentiation of CD4+ T cell subsets in rats.Int Immunopharmacol. 2019 May;70:313-323. Read more>>
  • Yao L et al. The proteasome activator REGγ counteracts immunoproteasome expression and autoimmunity. J Autoimmun. 2019 Jun 3. Read more>>
  • Bai, L et al. Renoprotective Effects of Artemisinin and Hydroxychloroquine Combination Therapy on IgA Nephropathy via Suppressing NF-κB Signaling and NLRP3 Inflammasome Activation by Exosomes in Rats. Biochemical Pharmacology. 2019 Aug22.Read more>>
  • Ruirui Luet al. Protective role of Astragaloside IV in chronic glomerulonephritis by activating autophagy through PI3K/AKT/AS160 pathway. Phytother Res. 2020 Dec;34(12):3236-3248.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat liver labeled with Anti-C3 Polyclonal Antibody, Unconjugated (bs-2934R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37°C


Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-C3 Polyclonal Antibody, Unconjugated (bs-2934R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) used at 1:200 dilution for 40 minutes at 37°C