bs-2955R [Primary Antibody]
C3a Receptor Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: C3a Receptor

Immunogen Range: 401-482/482


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 719

Swiss Prot: Q16581

Source: KLH conjugated synthetic peptide derived from human C3a Receptor/C3aR

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 54


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shinjyo, Noriko, et al. "Complement Peptide C3a Promotes Astrocyte Survival in Response to Ischemic Stress." Molecular Neurobiology (2015): 1-12.Read more>>
  • Ina Lackner. et al. Systemic and local cardiac inflammation after experimental long bone fracture, traumatic brain injury and combined trauma in mice. J Orthop Transl. 2021 May;28:39Read more>>
  • Tushar A. Shah. et al. Therapeutic Hypothermia Inhibits the Classical Complement Pathway in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy. Front Neurosci-Switz. 2021; 15: 616734Read more>>
  • Ina Lackner. et al. Reaming of femoral fractures with different reaming irrigator aspirator systems shows distinct effects on cardiac function after experimental polytrauma. J Orthop Res. 2020 Dec;38(12):2608-2618Read more>>
  • Liu, Tao. et al. Microarray-based analysis of renal complement components reveals a therapeutic target for lupus nephritis. Arthritis Res Ther. 2021 Dec;23(1):1-15Read more>>
  • Choi, Yun Ju. et al. Promotion of the inflammatory response in mid colon of complement component 3 knockout mice. Sci Rep-Uk. 2022 Feb;12(1):1-17Read more>>
  • Gong, Biao. et al. Complement C3a activates astrocytes to promote medulloblastoma progression through TNF-_. J NEUROINFLAMM. 2022 Dec;19(1):1-17Read more>>
  • Lackner Ina. et al. Cardiac alterations following experimental hip fracture - inflammaging as independent risk factor. FRONT IMMUNOL. 2022 Sep;0:4835Read more>>
VALIDATION IMAGES

293T lysates probed with C3a Receptor Polyclonal Antibody, Unconjugated (bs-2955R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


A549 lysates probed with C3a Receptor Polyclonal Antibody, Unconjugated (bs-2955R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Rat Spleen tissue lysates; Lane 2: Human A549 cell lysates; Lane 3: Human HL60 cell lysates; Lane 4: Human THP-1 cell lysates probed with C3a Receptor Polyclonal Antibody, Unconjugated (bs-2955R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.