Host: Rabbit
Target Protein: Alpha-ENaC
Immunogen Range: 201-300/669
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6337
Swiss Prot: P37088
Source: KLH conjugated synthetic peptide derived from human Alpha-ENaC
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 76
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
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- Yamamoto, Seiji, et al. "High salt loading induces urinary storage dysfunction via upregulation of epithelial sodium channel alpha in the bladder epithelium in Dahl salt-sensitive rats." Journal of Pharmacological Sciences (2017).Read more>>
- Scortegagna et al. The E3 ubiquitin ligase Siah1 regulates adrenal gland organization and aldosterone secretion. (2017) JCI.Insight. 2 Read more>>
- Zhen-zhenChen et al. Bu Yang Huan Wu Decoction prevents reperfusion injury following ischemic stroke in rats via inhibition of HIF-1 α, VEGF and promotion β-ENaC expression (2018)Journal of Ethnopharmacology Sep 13;228:70-81.Read more>>
- Chao Lu. et al. Inhibition of Pre-B Cell Colony Enhancing Factor Reduces Lung Injury in Rats Receiving Cardiopulmonary Bypass. Drug Des Dev Ther. 2021; 15: 51C6Read more>>
- Xiu Fei. et al. Aldosterone alleviates lipopolysaccharide-induced acute lung injury by regulating epithelial sodium channel through PI3K/Akt/SGK1 signaling pathway. Mol Cell Probe. 2021 Mar;:101709Read more>>
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- enedikt Fels. et al. Effects of Chronic Kidney Disease on Nanomechanics of the Endothelial Glycocalyx Are Mediated by the Mineralocorticoid Receptor. INT J MOL SCI. 2022 Jan;23(18):10659Read more>>
- Nami Obayashi. et al. Effect of irinotecan administration on amiloride-sensitive sodium taste responses in mice. EUR J ORAL SCI. 2023 Feb;131(2):e12922Read more>>
- Zheng Qian. et al. Inactivation of epithelial sodium ion channel molecules serves as effective diagnostic biomarkers in clear cell renal cell carcinoma. GENES GENOM. 2023 May;:1-12Read more>>
- Yu-qiong He. et al. Ursodeoxycholic acid alleviates sepsis-induced lung injury by blocking PANoptosis via STING pathway. INT IMMUNOPHARMACOL. 2023 Dec;125:111161Read more>>
Formalin-fixed and paraffin embedded rat kidney labeled with Anti-Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Rat kidney lysate at 40µg; Probed with Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:1000 overnight at 4°C followed by incubation with a conjugated secondary antibody at 1:20000 for 60 minutes at 37°C.
Mouse kidney lysate at 40µg; Probed with Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:1000 overnight at 4°C followed by incubation with a conjugated secondary antibody at 1:20000 for 60 minutes at 37°C.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Large intestine lysates probed with Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.