bs-2982R [Primary Antibody]
VIP Receptor 1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VIP Receptor 1

Immunogen Range: 351-457/457


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7433

Swiss Prot: P32241

Source: KLH conjugated synthetic peptide derived from human VIP Receptor 1/VAPC1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This is a receptor for VIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The affinity is VIP = PACAP-27 > PACAP-38.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 47


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ren, Xinxiu, et al. "Enteromorpha and polysaccharides from enteromorpha ameliorate loperamide-induced constipation in mice." Biomedicine & Pharmacotherapy (2017).Read more>>
  • Zeqi Tang. et al. Seasonal changes in the expression of PACAP, VPAC1, VPAC2, PAC1 and testicular activity in the testis of the muskrat (Ondatra zibethicus). EUR J HISTOCHEM. 2022 Mar 24; 66(2): 3398Read more>>
  • Lin Xia-Hui. et al. lncRNA-AC079061.1/VIPR1 axis may suppress the development of hepatocellular carcinoma: a bioinformatics analysis and experimental validation. J TRANSL MED. 2022 Dec;20(1):1-21Read more>>
VALIDATION IMAGES

Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti VIP Receptor 1/VAPC1 Polyclonal Antibody, Unconjugated (bs-2982R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 47kD. Observed band size: 47kD


Lane 1: Mouse Liver tissue lysates; Lane 2: Rat Liver tissue lysates; Lane 3: Human Raji cell lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Human FHC cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human MCF-7 cell lysates probed with VIP Receptor 1 Polyclonal Antibody, Unconjugated (bs-2982R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.