bs-2987R [Primary Antibody]
Vitamin D Receptor/VDR Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Vitamin D Receptor/VDR

Immunogen Range: 65-180/427


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7421

Swiss Prot: P11473

Source: KLH conjugated synthetic peptide derived from human Vitamin D Receptor

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 47


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Song, Zhixia, et al. "The PI3K/p-Akt Signaling Pathway participates in Calcitriol Ameliorating Podocyte Injury in DN Rats." Metabolism (2014).Read more>>
  • Tian, Yanyan, et al. "Effects of vitamin D on renal fibrosis in diabetic nephropathy model rats." International Journal of Clinical and Experimental Pathology 7.6 (2014): 3028-3037.Read more>>
  • Zhang, Xiaoliang, et al. "The novel role of TRPC6 in vitamin D ameliorating podocyte injury in STZ-induced diabetic rats." Molecular and cellular biochemistry (2014): 1-11.Read more>>
  • Wu, Hai-Yan, et al. "Serum 25-hydroxyvitamin D inversely associated with blood eosinophils in patients with persistent allergic rhinitis." Asia Pacific Allergy 7.4 (2017): 213.Read more>>
  • Zhang et al. 1,25-Dihydroxyvitamin D₃ Promotes High Glucose-Induced M1 Macrophage Switching to M2 via the VDR-PPARγ Signaling Pathway. (2015) Biomed.Res.In. 2015:157834Read more>>
  • "Jeremy M et al. Vitamin D3 mediated regulation of steroidogenesis mitigates testicular activity in an aged rat model.J Steroid Biochem Mol Biol. 2019 Jun;190:64-75."Read more>>
  • Bobek J et al. Onset of calciotropic receptors during the initiation of mandibular/alveolar bone formation. Ann Anat. 2019 Oct 12;227:151427.Read more>>
  • Guo Y et al. Calcitriol attenuates renal tubular epithelial cells apoptosis via inhibiting p38MAPK signaling in diabetic nephropathy. Acta Diabetol. 2020 Nov;57(11):1327-1335.Read more>>
  • Nianbin Song. et al. Multiple genetic programs contribute to CD4 T cell memory differentiation and longevity by maintaining T cell quiescence. Cell Immunol. 2020 Nov;357:10421Read more>>
  • Stenhouse Claire. et al. Novel Mineral Regulatory Pathways in Ovine Pregnancy: II. Calcium Binding Proteins, Calcium Transporters, and Vitamin D Signaling. Biol Reprod. 2021 ApRead more>>
  • Stenhouse Claire. et al. Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling. Biol Reprod. 2022 FebRead more>>
  • Natalia ?ab?d?. et al. Modulation of Fibroblast Activity via Vitamin D3 Is Dependent on Tumor Type—Studies on Mouse Mammary Gland Cancer. CANCERS. 2022 Jan;14(19):4585Read more>>
  • Stachowicz-Suhs Martyna. et al. Calcitriol promotes M2 polarization of tumor-associated macrophages in 4T1 mouse mammary gland cancer via the induction of proinflammatory cytokines. SCI REP-UK. 2024 Feb;14(1):1-2Read more>>
VALIDATION IMAGES

L1 rat brain lysates L2 rat kidney lysates probed with Anti Vitamin D Receptor/VDR Polyclonal Antibody, Unconjugated (bs-2987R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 47kD. Observed band size:47kD.\n


Formalin-fixed and paraffin embedded rat liver labeled with Anti-Vitamin D Receptor/VDR Polyclonal Antibody, Unconjugated (bs-2987R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Vitamin D Receptor/VDR Polyclonal Antibody, Unconjugated (bs-2987R) at 1:400 overnight at 4°C, DAB staining.


A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Vitamin D Receptor) polyclonal Antibody, Unconjugated (bs-2987R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.