Host: Rabbit
Target Protein: BMI1
Immunogen Range: 61-160/326
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 648
Swiss Prot: P35226
Source: KLH conjugated synthetic peptide derived from human Bmi1/PCGF4
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 37
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
Dog
Cow
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Penggang Liu. et al. Expression of tumour transcription factor GLI1 in canine mammary tumours tissue. VET MED SCI. 2022 Jun 06Read more>>
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti BMI1/PCGF4 Polyclonal Antibody, Unconjugated (bs-2999R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti BMI1/PCGF4 Polyclonal Antibody, Unconjugated (bs-2999R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti BMI1/PCGF4 Polyclonal Antibody, Unconjugated (bs-2999R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Human MCF-7 cell lysates probed with PCGF4/BMI1 Polyclonal Antibody, Unconjugated (bs-2999R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
NIH/3T3 cells were fixed with 4% PFA for 10min at room temperature , permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PCGF4/BMI1 Polyclonal Antibody(bs-2999R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
NIH/3T3 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PCGF4/BMI1 Polyclonal Antibody(bs-2999R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).