KLH conjugated synthetic phosphopeptide derived from human Bax around the phosphorylation site of Ser184
Purified by Protein A.
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
PRODUCT SPECIFIC PUBLICATIONS
- Park, Ga Bin, et al. "Melphalan-Induced Apoptosis of EBV-Transformed B Cells through Upregulation of TAp73 and XAF1 and Nuclear Import of XPA." The Journal of Immunology (2013): 1203442.Read more>>
- Gao, Xiu-Fang, et al. "Urocortin-2 suppression of p38-MAPK signaling as an additional mechanism for ischemic cardioprotection." Molecular and Cellular Biochemistry: 1-12.Read more>>
- Park, Gabin, et al. "Berberine induces mitochondrial apoptosis of EBV-transformed B cells through p53-mediated regulation of XAF1 and GADD45α." International Journal of Oncology.Read more>>
- Daverey, Amita, and Sandeep K. Agrawal. "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." Neuroscience 333 (2016): 92-103.Read more>>
Lane 1:mouse brain lysates probed with Rabbit Anti-Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Bax (Ser184) Polyclonal Antibody, Unconjugated (bs-3010R) at 1:200 overnight at 4°C, DAB staining.