bs-3016R [Primary Antibody]
ERK1/2(Thr202 + Tyr204) Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ERK1/2 Thr202 + Tyr204

Modification Site: Thr202 + Tyr204

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5595

Swiss Prot: P27361

Source: KLH conjugated synthetic phosphopeptide derived from human p44/42 MAPK around the phosphorylation site of (Thr202/Tyr204)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

p44/42 MAP Kinase(Thr202); ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 41


Cross Reactive Species: Human
Mouse
Rat
Dog
Others

Predicted Cross Reactive Species: Cow
Pig
Horse
Chicken
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Geng, Shanshan, et al. "Medium-chain triglyceride ameliorates insulin resistance and inflammation in high fat diet-induced obese mice." European Journal of Nutrition (2015): 1-10.Read more>>
  • Chen, Wei, et al. "Mycobacterium tuberculosis PE25/PPE41 protein complex induces activation and maturation of dendritic cells and drives Th2-biased immune responses." Medical Microbiology and Immunology (2015): 1-13.Read more>>
  • Wang, Xiao-yan, et al. "AMD3100 attenuates MMP-3 and MMP-9 expressions and prevents cartilage degradation in a monosodium iodoacetate-induced rat model of temporomandibular osteoarthritis." Journal of Oral and Maxillofacial Surgery (2016).Read more>>
  • Liu, Chen, et al. "Pulmonary artery denervation improves pulmonary arterial hypertension induced right ventricular dysfunction by modulating the local renin-angiotensin-aldosterone system." BMC Cardiovascular Disorders 16.1 (2016): 192.Read more>>
  • Liu, Lu, et al. "Anthraquinone derivative exerted hormetic effect on the apoptosis in oxygen-glucose deprivation-induced PC12 cells via ERK and Akt activated Nrf2/HO-1 signaling pathway." Chemico-Biological Interactions 262 (2017): 1-11.Read more>>
  • Zhang, Fengwei, et al. "Seasonal Expression of Oxytocin and Oxytocin Receptor in the Scented Gland of Male Muskrat (Ondatra zibethicus)." Scientific Reports 7.1 (2017): 16627.Read more>>
  • Chen et al. Downregulation of MACC1 expression enhances cisplatin sensitivity in SKOV-3/DDP cells. (2015) Genet.Mol.Re. 14:17134-44Read more>>
  • Tan et al. ART3 regulates triple-negative breast cancer cell function via activation of Akt and ERK pathways. (2016) Oncotarge. 7:46589-46602Read more>>
  • Zheng et al. Ginsenoside Rb1 improves cardiac function and remodeling in heart failure. (2017) Exp.Ani. 66:217-228Read more>>
  • Xing et al. Increased expression of receptor for advanced glycation end-products worsens focal brain ischemia in diabetic rats. (2012) Neural.Regen.Res. 7:1000-5Read more>>
  • Zhao X et al. Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway.(2018) Microb Pathog.125:431-437.Read more>>
  • Jeon et al. Activation of the complement system in an osteosarcoma cell line promotes angiogenesis through enhanced production of growth factors. (2018) Sci.Rep. 8:5415Read more>>
  • Fang et al. Upregulation of long noncoding RNA CCAT1-L promotes epithelial-mesenchymal transition in gastric adenocarcinoma. (2018) Onco.Targets.Ther. 11:5647-5655Read more>>
  • Li Y et al. Ecdysterone Accelerates Healing of Radiation-Induced Oral Mucositis in Rats by Increasing Matrix Cell Proliferation. Radiat Res. 2019 Mar;191(3):237-244. Read more>>
  • Fu S et al. Berberine suppresses mast cell-mediated allergic responses via regulating FcɛRI-mediated and MAPK signaling.Int Immunopharmacol. 2019 Jun;71:1-6. Read more>>
  • Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.Read more>>
  • Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523.Read more>>
  • Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.Read more>>
  • Liu F et al. Clinical and biological significances of heat shock protein 90 (Hsp90) in human nasopharyngeal carcinoma cells and anti-cancer effects of Hsp90 inhibitor. Biomed Pharmacother. 2019 Oct 18;120:109533. Read more>>
  • Zhang J et al. Down‐regulation of Suv39h1 attenuates neointima formation after carotid artery injury in diabetic rats. J Cell Mol Med. 2019 Nov 17. Read more>>
  • Gu F et al. Lactobacillus casei improves depression-like behavior in chronic unpredictable mild stress-induced rats by the BDNF-TrkB signal pathway and the intestinal microbiota. Food Funct. 2020 Jul 1;11(7):6148-6157.Read more>>
  • Lijing Wanget al. 1-(4-((5-chloro-4-((2-(isopropylsulfonyl)phenyl)amino)pyrimidin-2-yl)amino)-3-methoxyphenyl)-3-(2-(dimethylamino)ethyl)imidazolidin-2-one (ZX-42), a novel ALK inhibitor, induces apoptosis and protective autophagy in H2228 cells. J Pharm Pharmacol. 2020 Oct;72(10):1370-1382.Read more>>
  • Li X et al. Disseminated Melanoma Cells Transdifferentiate into Endothelial Cells in Intravascular Niches at Metastatic Sites. Cell Rep. 2020 Jun 16;31(11):107765.Read more>>
  • Chao Lu. et al. Inhibition of Pre-B Cell Colony Enhancing Factor Reduces Lung Injury in Rats Receiving Cardiopulmonary Bypass. Drug Des Dev Ther. 2021; 15: 51C6Read more>>
  • Yajing Lv. et al. Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway. Plos Biol. 2020 Nov;18(11):e3000872Read more>>
  • Xuejiao Zhou. et al. The novel ALK inhibitor ZX\29 induces apoptosis through inhibiting ALK and inducing ROS\mediated endoplasmic reticulum stress in Karpas299 cells. 2020 Nov 02Read more>>
  • Dejun Xu. et al. Melatonin protects mouse testes from palmitic acid\induced lipotoxicity by attenuating oxidative stress and DNA damage in a SIRT1\dependent manner. J Pineal Res. 2020 Nov;69(4):e1269Read more>>
  • Wenbo Ge. et al. 17-estradiol protects sheep oviduct epithelial cells against lipopolysaccharide-induced inflammation in vitro. Mol Immunol. 2020 Nov;127:21Read more>>
  • Xiaosu Chen. et al. DCBLD2 Mediates Epithelial-Mesenchymal Transition-Induced Metastasis by Cisplatin in Lung Adenocarcinoma. Cancers. 2021 Jan;13(6):1403Read more>>
  • Jingwen Zhou. et al. Zi Qi Decoction Alleviates Liver Fibrosis by Inhibiting the Toll-Like Receptor 4 (TLR4)-Related Nuclear Factor kappa b (NF-B) and Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways. Med Sci Monitor. 2021; 27: e929438-1Ce929438-12Read more>>
  • Qihe Tang. et al. Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-B Pathways Against Klebsiella pneumonia Infection. Front Pharmacol. 2021; 12: 651664Read more>>
  • Xiuhong Wang. et al. Protective effect of combination of anakinra and MCC950 against acute lung injury is achieved through suppression of the NF-B-mediated-MAPK and NLRP3-caspase pathways. Int Immunopharmacol. 2021 Aug;97:107506Read more>>
  • Jianye Yang. et al. Astragalus polysaccharide attenuates LPS-related inflammatory osteolysis by suppressing osteoclastogenesis by reducing the MAPK signalling pathway. 2021 Jun 02Read more>>
  • Ai Jin. et al. TSLP-induced collagen type-I synthesis through STAT3 and PRMT1 is sensitive to calcitriol in human lung fibroblasts. Bba-Mol Cell Res. 2021 Jun;:119083Read more>>
  • Lili Liu. et al. Rutin Ameliorates Cadmium-Induced Necroptosis in the Chicken Liver via Inhibiting Oxidative Stress and MAPK/NF-B Pathway. 2021 Jun 06Read more>>
  • Zhixia Jia. et al. Baicalin Ameliorates Chronic Unpredictable Mild Stress-Induced Depression through the BDNF/ERK/CREB Signaling Pathway. Behav Brain Res. 2021 Jul;:113463Read more>>
  • Ning Zhong. et al. Apatinib inhibits the growth of small cell lung cancer via a mechanism mediated by VEGF, PI3K/Akt and Ki-67/CD31. 2021 Sep 3Read more>>
  • Wei Liu. et al. Glycolysis and Reactive Oxygen Species Production Participate in T-2 Toxin-Stimulated Chicken Heterophil Extracellular Traps. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXXRead more>>
  • Zhikai Wu. et al. Fumonisin B1 induces chicken heterophil extracellular traps mediated by PAD4 enzyme and P2??1 receptor. Poultry Sci. 2021 Oct;:10155Read more>>
  • Tzu-Hsiang Lin. et al. A bilineage thermosensitive hydrogel system for stimulation of mesenchymal stem cell differentiation and enhancement of osteochondral regeneration. Compos Part B-Eng. 2022 Jan;:109614Read more>>
  • Zhang, Rongrong. et al. Compound traditional Chinese medicine dermatitis ointment ameliorates inflammatory responses and dysregulation of itch-related molecules in atopic dermatitis. Chin Med-Uk. 2022 Dec;17(1):1-19Read more>>
  • Lei Wang. et al. Lactobacillus plantarum DP189 Reduces _-SYN Aggravation in MPTP-Induced Parkinsons Disease Mice via Regulating Oxidative Damage, Inflammation, and Gut Microbiota Disorder. J Agr Food Chem. 2022;70(4):11631173Read more>>
  • Song, Yue. et al. Seasonal expression of extracellular signal regulated kinases in the colon of wild ground squirrels (Spermophilus dauricus). Mol Biol Rep. 2022 Jan;:1-7Read more>>
  • Linyan Cheng. et al. Icariin attenuates thioacetamide_induced bone loss via the RANKL_p38/ERK_NFAT signaling pathway. Mol Med Rep. 2022 Apr;25(4):1-11Read more>>
  • Yudan Zhao. et al. COX-2 is required to mediate crosstalk of ROS-dependent activation of MAPK/NF-_B signaling with pro-inflammatory response and defense-related NO enhancement during challenge of macrophage-like cell line with Giardia duodenalis. PLOS NEGLECT TROP D. 2022 Apr;16(4):e0010402Read more>>
  • Yanjie Wang. et al. Bisdemethoxycurcumin attenuates OVA_induced food allergy by inhibiting the MAPK and NF__B signaling pathways. EXP THER MED. 2022 Jun;23(6):1-8Read more>>
  • L Cheng. et al. Toxic effects of thioacetamide-induced femoral damage in New Zealand white rabbits by activating the p38/ERK signaling pathway. PHYSIOL RES. 2022 Apr 11Read more>>
  • Jiang, Liqiang. et al. Chicken heterophils extracellular traps act as early effectors against cyclopiazonic acid dependent upon NADPH oxidase, ROS and glycolysis. ARCH TOXICOL. 2022 May;:1-1Read more>>
  • Wen, Yukang. et al. Incomplete autophagy promotes the proliferation of Mycoplasma hyopneumoniae through the JNK and Akt pathways in porcine alveolar macrophages. VET RES. 2022 Dec;53(1):1-15Read more>>
  • Li, Manman. et al. Anti-inflammation of isoliquiritigenin via the inhibition of NF-B and MAPK in LPS-stimulated MAC-T cells. BMC VET RES. 2022 Dec;18(1):1-1Read more>>
VALIDATION IMAGES

Lane 1: human colon carcinoma lysates probed with Anti phospho-ERK1/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 44kD. Observed band size: 44kD


Formalin-fixed and paraffin embedded human liver labeled with Anti-phospho-ERK1/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-ERK1/2(Thr202 + Tyr204)Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37°C and DAPI staining


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 overnight at 4°C, DAB staining.


Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-ERK1/2 (Thr202 + Tyr204)) polyclonal Antibody, Unconjugated (bs-3016R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated (bs-2637R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.