bs-3020R [Primary Antibody]
p53BP1 (Ser25 + Ser29) Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: p53BP1 Ser25 + Ser29

Specificity: This phosphorylated site is the same in Mouse and homologous to p53BP1 Ser30 + Ser34 in Rat.

Modification Site: Ser25 + Ser29

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7158

Swiss Prot: Q12888

Source: KLH conjugated synthetic phosphopeptide derived from human p53BP1 around the phosphorylation site of Ser25/29

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Plays a key role in the response to DNA damage. May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 213


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cai, Zhiqiang, et al. "Oncogenic miR-17/20a Forms a Positive Feed-forward Loop with the p53 Kinase DAPK3 to Promote Tumorigenesis." Journal of Biological Chemistry (2015): jbc-M115.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti phospho-p53BP1(Ser25/29) Polyclonal Antibody, Unconjugated (bs-3020R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti phospho-p53BP1(Ser25/29) Polyclonal Antibody, Unconjugated (bs-3020R) at 1:200 followed by conjugation to the secondary antibody and DAB staining