bs-3031R [Primary Antibody]
ASK1 (Thr845) Polyclonal Antibody
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: ASK1 Thr845

Modification Site: Thr845

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 26408

Swiss Prot: O35099

Source: KLH conjugated synthetic phosphopeptide derived from mouse ASK1 around the phosphorylation site of Thr845

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 155


Cross Reactive Species: Human
Mouse
Rat
Bovine

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Taniuchi, Keisuke, et al. "Peroxiredoxin 1 Promotes Pancreatic Cancer Cell Invasion by Modulating p38 MAPK Activity." Pancreas (2014).Read more>>
  • Choi H et al. Apoptosis signal-regulating kinase 1 activation by Nox1-derived oxidants is required for TNFα receptor endocytosis. Am J Physiol Heart Circ Physiol. 2019 Mar 29.Read more>>
  • Zhou D et al. Inhibition of apoptosis signal-regulating kinase by paeoniflorin attenuates neuroinflammation and ameliorates neuropathic pain. J Neuroinflammation. 2019 Apr 11;16(1):83.Read more>>
  • Li T et al. ASK1 phosphorylation regulates astrocytic reactive gliosis in vitro and in vivo. Neurosci Lett. 2020 Jan 18;716:134675.Read more>>
  • Ying-Jung Hsu. et al. Protective Effect of Fenofibrate on Oxidative Stress-Induced Apoptosis in RetinalCChoroidal Vascular Endothelial Cells: Implication for Diabetic Retinopathy Treatment. Antioxidants-Basel. 2020 Aug;9(8):712Read more>>
  • Xiaoli Guo. et al. ASK1 signaling regulates phase-specific glial interactions during neuroinflammation. P Natl Acad Sci Usa. 2022 Feb;119(6)Read more>>
  • Wen Jiang. et al. Hirsutine ameliorates myocardial ischemia-reperfusion injury through improving mitochondrial function via CaMKII pathway. CLIN EXP HYPERTENS. 2023;45(1):Article: 2192444Read more>>
VALIDATION IMAGES

L1 rat heart, L2 rat brain lysates probed (bs-3031R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 155kDa.


Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti-phospho-ASK1(Thr845) Polyclonal Antibody, Unconjugated (bs-3031R) at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining


Lane 1: Rat Cerebrum lysates; Lane 2: Rat Stomach lysates probed with phospho-ASK1 Polyclonal Antibody, Unconjugated (bs-3031R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ASK1 (Thr845) Polyclonal Antibody(bs-3031R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).