Host: Rabbit
Target Protein: CD19 Tyr531
Modification Site: Tyr531
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 930
Swiss Prot: P15391
Source: KLH conjugated synthetic phosphopeptide derived from human CD19 around the phosphorylation site of Tyr531 [DS(p-Y)EN]
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 59
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
Pig
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
HL-60 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD19(Tyr531) Antibody（bs-3065R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).