Host: Rabbit
Target Protein: CaMK2 Tyr231
Specificity: This phosphorylation site is homologous to Tyr231 in CaMK2B and CaMK2D and will react with these proteins when phosphorylated at this site.
Modification Site: Tyr230
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 815
Swiss Prot: Q9UQM7
Source: KLH conjugated synthetic phosphopeptide derived from human CaMK2 alpha around the phosphorylation site of Tyr230
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 54
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Henrique Ballassini Abdalla et al. Activation of PPAR- induces macrophage polarization and reduces neutrophil migration mediated by heme oxygenase 1. Int Immunopharmacol. 2020 Jul;84:106565.Read more>>
- ei Miao. et al. Protective effect of HCN2-induced SON sensitization on chronic visceral hypersensitivity in neonatal-CRD rat model. Brain Res. 2021 Sep;1767:147538Read more>>
- He Zhang. et al. Effect of feeding regimen on circadian activity rhythms of food anticipatory by ghrelin hormone in a pig model. 2022 Mar 05Read more>>
- Qi Sun. et al. MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma. CANCER LETT. 2022 Jul;537:215678Read more>>
- Shasha Chen. et al. Quercetin alleviates zearalenone-induced apoptosis and necroptosis of porcine renal epithelial cells by inhibiting CaSR/CaMKII signaling pathway. FOOD CHEM TOXICOL. 2023 Nov;:114184Read more>>
- Hailin Liu. et al. Dexamethasone upregulates macrophage PIEZO1 via SGK1, suppressing inflammation and increasing ROS and apoptosis. BIOCHEM PHARMACOL. 2024 Apr;222:11605Read more>>
Formalin-fixed and paraffin embedded rat brain labeled with Anti-phospho-CaMK2 alpha (Tyr231)Polyclonal Antibody, Unconjugated (bs-3081R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-phospho-CaMK2 alpha (Tyr231)Polyclonal Antibody, Unconjugated (bs-3081R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-CaMK2 alpha(Tyr230)) Polyclonal Antibody, Unconjugated (bs-3081R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-CaMK2 alpha(Tyr230)) Polyclonal Antibody, Unconjugated (bs-3081R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-CaMK2 alpha(Tyr230)) Polyclonal Antibody, Unconjugated (bs-3081R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Muscle tissue lysates; Lane 2: Mouse Heart tissue lysates probed with phospho-CaMK2 alpha (Tyr230) Polyclonal Antibody, Unconjugated (bs-3081R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.