bs-3099R [Primary Antibody]
Cofilin (Ser3) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cofilin Ser3

Specificity: This phosphorylation site is homologous in the listed cross reactive species at the specified location.

Modification Site: Ser3

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1072

Swiss Prot: P23528

Source: KLH conjugated synthetic phosphopeptide derived from human Cofilin around the phosphorylation site of Ser3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 18


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Cow
Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • "Yee MC et al.Protease-activated Receptor-2 Signaling through β-Arrestin-2 Mediates Alternaria Alkaline Serine Protease-induced Airway Inflammation.(2018)Am J Physiol Lung Cell Mol Physiol.Oct 18. "Read more>>
  • Xue Y et al. Methamphetamine reduces expressions of tight junction proteins, rearranges F-actincytoskeleton and increases the blood brain barrier permeability via the RhoA/ROCK-dependent pathway. Biochem Biophys Res Commun. 2019 Feb 5;509(2):395-401.Read more>>
  • Wufuer R et al. Downregulation of Rac1/PAK1/LIMK1/cofilin signaling pathway in colon cancer SW620 cells treated with Chlorin e6 photodynamic therapyPhotodiagnosis Photodyn Ther.2020 Dec 8;102143.Read more>>
  • Nuo Heng. et al. RhoA improves cryopreservation of rooster sperm through the Rho/RhoA-associated Kinase/cofilin pathway. POULTRY SCIENCE. 2022 Jul;:102051Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti Phospho-Cofilin (Ser3) Polyclonal Antibody (bs-3099R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining