bs-3138R [Primary Antibody]
FoxO3a (Ser318 + Ser321) Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FoxO3a Ser318 + Ser321

Specificity: These phosphorylation sites are homologous to Ser317 + Ser320 in Mouse and Rat. Due to the highly conserved nature of this sequence, there is a chance that this antibody will react with FoxO1a when phosphorylated at the the sites of Ser322 and Ser325 in Human, Ser319 and Ser322 in Mouse, and Ser316 and Ser319 in Rat.

Modification Site: Ser318 + Ser321

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2309

Swiss Prot: O43524

Source: KLH conjugated synthetic phosphopeptide derived from human FoxO3a around the phosphorylation site of Ser318/321

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34C expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 71


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Sheep
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Mingkun Zhu. et al. MAPK, AKT/FoxO3a and mTOR pathways are involved in cadmium regulating the cell cycle, proliferation and apoptosis of chicken follicular granulosa cells. Ecotox Environ Safe. 2021 May;214:112091Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-Phospho-FoxO3a (Ser318/321) Polyclonal Antibody, Unconjugated (bs-3138R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FoxO3a (Ser318 + Ser321) Polyclonal Antibody, Unconjugated (bs-3138R) at 1:400 overnight at 4°C, DAB staining.