bs-3155R-Cy5.5 [Conjugated Primary Antibody]
GCN2 (Thr899) Polyclonal Antibody, Cy5.5 Conjugated
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: GCN2 Thr899

Specificity: This phosphorylation site is homologous to Thr898 in Mouse and Thr899 in Rat.

Modification Site: Thr899

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 440275

Swiss Prot: Q9P2K8

Source: KLH conjugated synthetic phosphopeptide derived from human GCN2 around the phosphorylation site of Thr899

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses.

Conjugation: Cy5.5

Excitation/ Emission: 675nm/694nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 189


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lehman, Stacey L., Sandra Ryeom, and Constantinos Koumenis. "Signaling through alternative Integrated Stress Response pathways compensates for GCN2 loss in a mouse model of soft tissue sarcoma." Scientific Reports 5 (2015).Read more>>
  • Changzheng Li. et al. Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2_ axis. CELL STEM CELL. 2022 Jul;29:1119Read more>>
VALIDATION IMAGES