bs-3155R-FITC [Conjugated Primary Antibody]
Phospho-GCN2 (Thr899) Polyclonal Antibody, FITC Conjugated
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Host: Rabbit

Target Protein: Phospho-GCN2 (Thr899)

Specificity: This phosphorylation site is homologous to Thr898 in Mouse and Thr899 in Rat.

Modification Site: Thr899

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 440275

Swiss Prot: Q9P2K8

Source: KLH conjugated synthetic phosphopeptide derived from human GCN2 around the phosphorylation site of Thr899

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)

Predicted Molecular Weight: 189


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lehman, Stacey L., Sandra Ryeom, and Constantinos Koumenis. "Signaling through alternative Integrated Stress Response pathways compensates for GCN2 loss in a mouse model of soft tissue sarcoma." Scientific Reports 5 (2015).Read more>>
  • Changzheng Li. et al. Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2_ axis. CELL STEM CELL. 2022 Jul;29:1119Read more>>
  • Zikang Xing. et al. IDO1 Inhibitor RY103 Suppresses Trp-GCN2-Mediated Angiogenesis and Counters Immunosuppression in Glioblastoma. PHARMACEUTICS. 2024 Jul;16(7):87Read more>>
VALIDATION IMAGES

Molt-4cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GCN2 (Thr899) Polyclonal Antibody(bs-3155R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).