bs-3159R-A647

DATASHEET

Host: Rabbit

Target Protein: FAK Tyr397

Specificity: This phosphorylation site is homologous to that of Tyr428 in Mouse and Tyr397 in Rat.

Modification Site: Tyr397

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5747

Swiss Prot: Q05397

Source: KLH cunjugated synthetic phosphopeptide derived from human FAK 1 around the phosphorylation site of Tyr397

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq, Dec 2010]

Conjugation: ALEXA FLUOR® 647

Excitation/ Emission: 650nm/665nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 116

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.