bs-3165R [Primary Antibody]
Phospho-Gab1 (Tyr627) Polyclonal Antibody
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Host: Rabbit

Target Protein: Phospho-Gab1 (Tyr627)

Modification Site: Tyr627

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2549

Swiss Prot: Q13480

Source: KLH conjugated Synthesised phosphopeptide derived from human Gab1 around the phosphorylation site of Tyr627

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c Met), and epidermal growth factor receptor (EGF), as well as in response to cell cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3 kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 76


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Phospho-Gab1 (Tyr627) polyclonal antibody, unconjugated (bs-3165R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.