bs-3185R
[Primary Antibody]
Histone H2A.X (Ser140) Antibody
DATASHEET
Host: Rabbit
Target Protein: Histone H2A.X Ser140
Specificity: This phosphorylation site is homologous to that of Ser140 in Mouse and Rat.
Modification Site: Ser140
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3014
Swiss Prot: P16104
Source: KLH conjugated synthetic phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser140
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 16
Cross Reactive Species:
Human
Others
Predicted Cross Reactive Species:
Mouse
Rat
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Song, Xiufang, et al. "An unpredicted downregulation of RAD51 suggests genome instability induced by tetrachlorobenzoquinone." Chemical Research in Toxicology (2016).Read more>>
- Shi et al. Dihydroartemisinin induces autophagy-dependent death in human tongue squamous cell carcinoma cells through DNA double-strand break-mediated oxidative stress. (2017) Oncotarget. 8:45981-45993Read more>>
- Wang L et al. Antimalarial Dihydroartemisinin Triggers Autophagy within HeLa Cells of Human Cervical Cancer through Bcl-2 Phosphorylation at Ser70. (2018) Phytomedicine. 7113(18)30503-8Read more>>
- Chen X et al. Reactive oxygen species induced by icaritin promote DNA strand breaks and apoptosis in human cervical cancer cells.(2019)Oncol Rep. Feb;41(2):765-778.Read more>>
- Zhang Q et al. Photoactivatable Prodrug-Backboned Polymeric Nanoparticles for Efficient Light-Controlled Gene Delivery and Synergistic Treatment of Platinum-Resistant Ovarian Cancer. Nano Lett. 2020 Apr 9. Read more>>
- Liu Yahong. et al. Preclinical Evaluation of Safety, Pharmacokinetics, Efficacy, and Mechanism of Radioprotective Agent HL-003. Oxid Med Cell Longev. 2021;2021:6683836Read more>>
- Sun Yuhuan. et al. Near-infrared-traceable DNA nano-hydrolase: specific eradication of telomeric G-overhang in vivo. Nucleic Acids Res. 2020 Sep;48(17):9986-9994Read more>>
- Donglin Xia. et al. AuCHemoglobin Loaded Platelet Alleviating Tumor Hypoxia and Enhancing the Radiotherapy Effect with Low-Dose X-ray. Acs Nano. 2020;14(11):15654C15668Read more>>
- Li na Wang. et al. Fighting against Drug\Resistant Tumors by Inhibition of -Glutamyl Transferase with Supramolecular Platinum Prodrug Nano-Assemblies. 2021 May 06Read more>>
- Yuan SJ. et al. Conjugation with nanodiamonds via hydrazone bond fundamentally alters intracellular distribution and activity of doxorubicin.. Int J Pharmaceut. 2021 Jul;606:120872-120872Read more>>
- Ning Han. et al. Ferroptosis triggered by dihydroartemisinin facilitates chlorin e6 induced photodynamic therapy by inhibiting GPX4 and enhancing ROS. Eur J Pharmacol. 2022 Feb;:174797Read more>>
- Yu TT. et al. Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING.. Front Pharmacol. 2022 Mar;13:837784-837784Read more>>
- Yang, Xiao-Xin. et al. A nanoreactor boosts chemodynamic therapy and ferroptosis for synergistic cancer therapy using molecular amplifier dihydroartemisinin. J NANOBIOTECHNOL. 2022 Dec;20(1):1-19Read more>>
- Liu-Gen Li. et al. Dihydroartemisinin remodels macrophage into an M1 phenotype via ferroptosis-mediated DNA damage. FRONT PHARMACOL. 2022; 13: 949835Read more>>
- Siyun Lei. et al. PARP inhibitors intervene DNA damage repair for the enhancement of tumor photodynamic therapy. PHOTODIAGN PHOTODYN. 2022 Aug;:103058Read more>>
- Ning Han. et al. Dihydroartemisinin elicits immunogenic death through ferroptosis-triggered ER stress and DNA damage for lung cancer immunotherapy. PHYTOMEDICINE. 2023 Jan;:154682Read more>>
- Ting-Ting Yu. et al. Chlorin e6-induced photodynamic effect facilitates immunogenic cell death of lung cancer as a result of oxidative endoplasmic reticulum stress and DNA damage. INT IMMUNOPHARMACOL. 2023 Feb;115:109661Read more>>
- Yuan-Jian Hui. et al. Up-regulation of ABCG2 by MYBL2 deletion drives Chlorin e6-mediated photodynamic therapy resistance in colorectal cancer. PHOTODIAGN PHOTODYN. 2023 Apr;:103558Read more>>
- Liu-Gen Li. et al. A Dihydroartemisinin-Loaded Nanoreactor Motivates Anti-Cancer Immunotherapy by Synergy-Induced Ferroptosis to Activate Cgas/STING for Reprogramming of Macrophage. ADV HEALTHC MATER. 2023 Aug;:2301561Read more>>
- Zi-Yi Yang. et al. Cepharanthine synergizes with photodynamic therapy for boosting ROS-driven DNA damage and suppressing MTH1 as a potential anti-cancer strategy. PHOTODIAGN PHOTODYN. 2023 Nov;:103917Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Anti-Phospho-Histone H2A.X (Ser139) Polyclonal Antibody, Unconjugated (bs-3185R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Hela cell lysates probed with Histone H2A.X (Ser139) Polyclonal Antibody, Unconjugated (bs-3185R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Histone H2A.X (Ser140) Antibody(bs-3185R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse BV2 cell lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human 293T cell lysates; Lane 4: Human Jurkat cell lysates probed with Phospho-H2AX (Ser139) Polyclonal Antibody, Unconjugated (bs-3185R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.