bs-3185R-A350 [Conjugated Primary Antibody]
Histone H2A.X (Ser140) Antibody, ALEXA FLUOR® 350 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Histone H2A.X Ser140

Specificity: This phosphorylation site is homologous to that of Ser140 in Mouse and Rat.

Modification Site: Ser140

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3014

Swiss Prot: P16104

Source: KLH conjugated synthetic phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser140

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Conjugation: ALEXA FLUOR® 350

Excitation/ Emission: 346nm/442nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 16


Cross Reactive Species: Human
Others

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Song, Xiufang, et al. "An unpredicted downregulation of RAD51 suggests genome instability induced by tetrachlorobenzoquinone." Chemical Research in Toxicology (2016).Read more>>
  • Shi et al. Dihydroartemisinin induces autophagy-dependent death in human tongue squamous cell carcinoma cells through DNA double-strand break-mediated oxidative stress. (2017) Oncotarget. 8:45981-45993Read more>>
  • Wang L et al. Antimalarial Dihydroartemisinin Triggers Autophagy within HeLa Cells of Human Cervical Cancer through Bcl-2 Phosphorylation at Ser70. (2018) Phytomedicine. 7113(18)30503-8Read more>>
  • Chen X et al. Reactive oxygen species induced by icaritin promote DNA strand breaks and apoptosis in human cervical cancer cells.(2019)Oncol Rep. Feb;41(2):765-778.Read more>>
  • Zhang Q et al. Photoactivatable Prodrug-Backboned Polymeric Nanoparticles for Efficient Light-Controlled Gene Delivery and Synergistic Treatment of Platinum-Resistant Ovarian Cancer. Nano Lett. 2020 Apr 9. Read more>>
  • Liu Yahong. et al. Preclinical Evaluation of Safety, Pharmacokinetics, Efficacy, and Mechanism of Radioprotective Agent HL-003. Oxid Med Cell Longev. 2021;2021:6683836Read more>>
  • Sun Yuhuan. et al. Near-infrared-traceable DNA nano-hydrolase: specific eradication of telomeric G-overhang in vivo. Nucleic Acids Res. 2020 Sep;48(17):9986-9994Read more>>
  • Donglin Xia. et al. AuCHemoglobin Loaded Platelet Alleviating Tumor Hypoxia and Enhancing the Radiotherapy Effect with Low-Dose X-ray. Acs Nano. 2020;14(11):15654C15668Read more>>
  • Li na Wang. et al. Fighting against Drug\Resistant Tumors by Inhibition of -Glutamyl Transferase with Supramolecular Platinum Prodrug Nano-Assemblies. 2021 May 06Read more>>
  • Yuan SJ. et al. Conjugation with nanodiamonds via hydrazone bond fundamentally alters intracellular distribution and activity of doxorubicin.. Int J Pharmaceut. 2021 Jul;606:120872-120872Read more>>
  • Ning Han. et al. Ferroptosis triggered by dihydroartemisinin facilitates chlorin e6 induced photodynamic therapy by inhibiting GPX4 and enhancing ROS. Eur J Pharmacol. 2022 Feb;:174797Read more>>
  • Yu TT. et al. Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING.. Front Pharmacol. 2022 Mar;13:837784-837784Read more>>
  • Yang, Xiao-Xin. et al. A nanoreactor boosts chemodynamic therapy and ferroptosis for synergistic cancer therapy using molecular amplifier dihydroartemisinin. J NANOBIOTECHNOL. 2022 Dec;20(1):1-19Read more>>
  • Liu-Gen Li. et al. Dihydroartemisinin remodels macrophage into an M1 phenotype via ferroptosis-mediated DNA damage. FRONT PHARMACOL. 2022; 13: 949835Read more>>
  • Siyun Lei. et al. PARP inhibitors intervene DNA damage repair for the enhancement of tumor photodynamic therapy. PHOTODIAGN PHOTODYN. 2022 Aug;:103058Read more>>
  • Ning Han. et al. Dihydroartemisinin elicits immunogenic death through ferroptosis-triggered ER stress and DNA damage for lung cancer immunotherapy. PHYTOMEDICINE. 2023 Jan;:154682Read more>>
  • Ting-Ting Yu. et al. Chlorin e6-induced photodynamic effect facilitates immunogenic cell death of lung cancer as a result of oxidative endoplasmic reticulum stress and DNA damage. INT IMMUNOPHARMACOL. 2023 Feb;115:109661Read more>>
  • Yuan-Jian Hui. et al. Up-regulation of ABCG2 by MYBL2 deletion drives Chlorin e6-mediated photodynamic therapy resistance in colorectal cancer. PHOTODIAGN PHOTODYN. 2023 Apr;:103558Read more>>
  • Liu-Gen Li. et al. A Dihydroartemisinin-Loaded Nanoreactor Motivates Anti-Cancer Immunotherapy by Synergy-Induced Ferroptosis to Activate Cgas/STING for Reprogramming of Macrophage. ADV HEALTHC MATER. 2023 Aug;:2301561Read more>>
  • Zi-Yi Yang. et al. Cepharanthine synergizes with photodynamic therapy for boosting ROS-driven DNA damage and suppressing MTH1 as a potential anti-cancer strategy. PHOTODIAGN PHOTODYN. 2023 Nov;:103917Read more>>
VALIDATION IMAGES