bs-3187R [Primary Antibody]
Phospho-Histone H3 (Ser28) Polyclonal Antibody
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Host: Rabbit

Target Protein: Phospho-Histone H3 (Ser28)

Specificity: This phosphorylation site is homologous across the listed species.

Modification Site: Ser28

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8290

Swiss Prot: Q16695

Source: KLH conjugated synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser29

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 15


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Cow
Pig
Rabbit
Fruit Fly

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Phospho-Histone H3 (Ser28) Polyclonal Antibody, Unconjugated (bs-3187R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Hela cells were fixed with 70% ice-cold methanol overnight at 4℃，permeabilized with 90% ice-cold methanol for 20 min at -20℃ , and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Histone H3 (Ser29) Polyclonal Antibody at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Control cells (dark blue), secondary only (light blue), Isotype control (orange) and Histone H3 (Ser29) (green).