bs-3195R [Primary Antibody]
IRF3 (Ser396) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IRF3 Ser396

Modification Site: Ser396

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3661

Swiss Prot: Q14653

Source: KLH conjugated synthetic phosphopeptide derived from human IRF3 around the phosphorylation site of Ser396

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 47


Cross Reactive Species: Human
Mouse
Rat
Monkey

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ichikawa, Tomohiro, et al. "TLR3 Activation Augments Matrix Metalloproteinase Production through Reactive Nitrogen Species Generation in Human Lung Fibroblasts." The Journal of Immunology (2014): 1302919.Read more>>
  • Le Bel, Manon, and Jean Gosselin. "Leukotriene B 4 Enhances NOD2-Dependent Innate Response against Influenza Virus Infection." PloS one 10.10 (2015): e0139856.Read more>>
  • Ando, Makoto, et al. "Poly (I: C) impairs NO donor-induced relaxation by overexposure to NO via the NF-kappa B/iNOS pathway in rat superior mesenteric arteries." Free Radical Biology and Medicine (2017).Read more>>
  • Egarnes, Benoit, Marie-Renée Blanchet, and Jean Gosselin. "Treatment with the NR4A1 agonist cytosporone B controls influenza virus infection and improves pulmonary function in infected mice." PLOS ONE 12.10 (2017): e0186639.Read more>>
  • Menasria et al. Both TRIF and IPS-1 adaptor proteins contribute to the cerebral innate immune response against herpes simplex virus 1 infection. (2013) J.Virol. 87:7301-8Read more>>
  • Egarnes et al. Treatment with the NR4A1 agonist cytosporone B controls influenza virus infection and improves pulmonary function in infected mice. (2017) PLoS.One. 12:e0186639Read more>>
  • Wan B et al. Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction. Int J Biol Macromol. 2019 Jul 5;138:198-206. Read more>>
  • H.Q. Hu. et al. The STING-IRF3 pathway is involved in lipotoxic injury of pancreatic cells in type 2 diabetes. Mol Cell Endocrinol. 2020 Dec;518:11089Read more>>
VALIDATION IMAGES

Mouse liver lysates probed with Anti-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.


U251 lysates probed with IRF3 (Ser396) Polyclonal Antibody, unconjugated (bs-3195R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Heart tissue lysates; Lane 4: Rat Testis tissue lysates probed with Phospho-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.