bs-3196R-A488 [Conjugated Primary Antibody]
IRF7 (Ser471 + Ser472) Antibody, ALEXA FLUOR® 488 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IRF7 Ser471 + Ser472

Modification Site: Ser471 + Ser472

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3665

Swiss Prot: Q92985

Source: KLH conjugated synthetic phosphopeptide derived from human IRF7 around the phosphorylation site of Ser471/472

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.

Conjugation: ALEXA FLUOR® 488

Excitation/ Emission: 490nm/525nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 54


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Han, Young Woo, et al. " Distinct Dictation of Japanese Encephalitis Virus-Induced Neuroinflammation and Lethality via Triggering TLR3 and TLR4 Signal Pathways." PLoS Pathogens 10(9) (2014): e103882.Read more>>
  • Le Bel, Manon, and Jean Gosselin. "Leukotriene B 4 Enhances NOD2-Dependent Innate Response against Influenza Virus Infection." PloS one 10.10 (2015): e0139856.Read more>>
  • Egarnes, Benoit, Marie-Renée Blanchet, and Jean Gosselin. "Treatment with the NR4A1 agonist cytosporone B controls influenza virus infection and improves pulmonary function in infected mice." PLOS ONE 12.10 (2017): e0186639.Read more>>
  • Menasria et al. Both TRIF and IPS-1 adaptor proteins contribute to the cerebral innate immune response against herpes simplex virus 1 infection. (2013) J.Virol. 87:7301-8Read more>>
  • Egarnes et al. Treatment with the NR4A1 agonist cytosporone B controls influenza virus infection and improves pulmonary function in infected mice. (2017) PLoS.One. 12:e0186639Read more>>
  • Peng K. et al. Transcriptome Analyses of Senecavirus A-Infected PK-15 Cells: RIG-I and IRF7 Are the Important Factors in Inducing Type III Interferons.. Front Microbiol. 2022 Mar;13:846343-846343Read more>>
  • Anette Christ. et al. Low Density Lipoprotein Exposure of Plasmacytoid Dendritic Cells Blunts Toll-like Receptor 7/9 Signaling via NUR77. BIOMEDICINES. 2022 May;10(5):1152Read more>>
  • HuiDan Deng. et al. Mfn2 is responsible for inhibition of the RIG-I/IRF7 pathway and activation of NLRP3 inflammasome in Seneca Valley virus-infected PK-15 cells to promote viral replication. FRONT IMMUNOL. 2022; 13: 955671Read more>>
  • Jianhao Yang. et al. Serum amyloid A regulates TLR2/4-mediated IFN- signaling pathway against Marek's disease virus. VIRUS RES. 2023 Jan;:199044Read more>>
  • Dadi Peng. et al. PPM1G regulates hepatic ischemia/reperfusion injury through STING-mediated inflammatory pathways in macrophages. IMMUN INFLAMM DIS. 2024 Feb;12(2):e1189Read more>>
VALIDATION IMAGES