Host: Rabbit
Target Protein: IRS1 Tyr612
Specificity: This phosphorylation site is homologous to that of Tyr608 in Mouse and Rat.
Modification Site: Tyr612
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3667
Swiss Prot: P35568
Source: KLH conjugated synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of Tyr612 [DG(p-Y)MP]
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 132
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
Dog
Cow
Pig
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
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Formalin-fixed and paraffin embedded human hepatocellular carcinoma labeled with Anti phospho-IRS1(Ser612) Polyclonal Antibody, Unconjugated (bs-3200R) at 1:200 followed by conjugation to the secondary antibody
Hela cells were fixed with 70% ice-cold methanol overnight at 4℃，0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRS1(Tyr612) Polyclonal Antibody at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed bysecondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Control cells (dark blue), secondary only (light blue), Isotype control (orange) and IRS1(Tyr612) (green)