bs-3208R-Biotin

DATASHEET

Host: Rabbit

Target Protein: Phospho-Jak3 (Tyr980 + Tyr981)

Specificity: These phosphorylation sites are homologous to Tyr976 + Tyr977 in Mouse and Rat.

Modification Site: Tyr980 + Tyr981

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3718

Swiss Prot: P52333

Source: KLH conjugated synthetic phosphopeptide derived from human Jak3 around the phosphorylation site of Tyr980/981

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100µL

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)

Predicted Molecular Weight: 125

Predicted Cross Reactive Species: Human
Mouse
Rat
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.