bs-3220R [Primary Antibody]
HER4 (Tyr1284) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HER4 Tyr1284

Modification Site: Tyr1284

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2066

Swiss Prot: Q15303

Source: KLH conjugated synthetic phosphopeptide derived from human HER4 around the phosphorylation site of Tyr1284

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 142


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xin-yu Qiao. et al. Involvement of CXCL10 in Neuronal Damage under the Condition of Spinal Cord Injury and the Potential Therapeutic Effect of Nrg1. J INTEGR NEUROSCI. 2023 Jul;22(4):96Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin-embedded Human Mammary Gland tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with HER4 (Tyr1284) Polyclonal Antibody, Unconjugated (bs-3220R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody, DAB and counterstaining.


Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with HER4 (Tyr1284) Polyclonal Antibody, Unconjugated (bs-3220R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody, DAB and counterstaining.


Paraformaldehyde-fixed, paraffin-embedded Mouse Pancreas tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with HER4 (Tyr1284) Polyclonal Antibody, Unconjugated (bs-3220R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody, DAB and counterstaining.