bs-3250R [Primary Antibody]
LKB1(Thr189) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LKB1 Thr189

Modification Site: Thr189

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6794

Source: KLH conjugated synthetic phosphopeptide derived from human LKB1 around the phosphorylation site of Thr189 [G(p-T)LK]

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 48


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xiang Li. et al. The novel ginsenoside AD2 prevents angiotensin II-induced connexin 40 and connexin 43 dysregulation by activating AMP kinase signaling in perfused beating rat atria. Chem-Biol Interact. 2021 Mar;:10943Read more>>
  • Zuodong Chen. et al. Oxidative stress induced by hydrogen peroxide promotes glycolysis by activating CaMKK/LKB1/AMPK pathway in broiler breast muscle. Poultry Sci. 2021 Dec;:101681Read more>>
  • Xu Jiawen. et al. Dietary creatine nitrate enhances muscle creatine loading and delays postmortem glycolysis of broilers that experienced preslaughter transport. J ANIM SCI. 2022 AugRead more>>
VALIDATION IMAGES

A431 lysates probed with LKB1(Thr189) Polyclonal Antibody, Unconjugated (bs-3250R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Human K562 cell lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human U937 cell lysates probed with LKB1 Polyclonal Antibody, Unconjugated (bs-3250R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.