Host: Rabbit
Target Protein: Lyn Tyr397
Specificity: Due to the highly conserved nature of this protein, this antibody will react with the related proteins LCK(Tyr394), Src(Tyr418), and YES1(Tyr426) if phosphorylated at this site due to homology.
Modification Site: Tyr397
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4067
Swiss Prot: P07948
Source: KLH conjugated synthetic phosphopeptide derived from human Lyn around the phosphorylation site of Tyr397
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 56
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Pig
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- He S et al. Avian leukosis virus subgroup J induces B cell anergy mediated by Lyn inhibited BCR signal transduction. Vet Microbiol. 2020 Aug;247:108781.Read more>>
- Xiangyu Liang. et al. Effects and potential mechanisms of Saposhnikovia divaricata (Turcz.) Schischk. On type I allergy and pseudoallergic reactions in vitro and in vivo. J ETHNOPHARMACOL. 2024 Jan;318:116942Read more>>
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-Lyn (Tyr396) Polyclonal Antibody, Unconjugated (bs-3257R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse placenta lysates; Lane 2: Mouse lung lysates probed with Lyn (Tyr397) Polyclonal Antibody, Unconjugated (bs-3257R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lyn (Tyr397) Polyclonal Antibody, Unconjugated (bs-3257R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Lyn (Tyr397)) Polyclonal Antibody, Unconjugated (bs-3257R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Lyn (Tyr397)) Polyclonal Antibody, Unconjugated (bs-3257R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Lyn (Tyr397)) Polyclonal Antibody, Unconjugated (bs-3257R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Embryo tissue lysates; Lane 3: Mouse BV2 cell lysates; Lane 4: Mouse Raw264.7 cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human Raji cell lysates probed with Phospho-Lyn (Tyr397) Polyclonal Antibody, Unconjugated (bs-3257R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.