VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-MKK3(Ser218)/MKK6(Ser207) Polyclonal Antibody, Unconjugated (bs-3274R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-MKK3(Ser218)/MKK6(Ser207) Polyclonal Antibody, Unconjugated (bs-3274R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Mouse muscle lysates probed with MKK3(Ser218) + MKK6(Ser207) Polyclonal Antibody, Unconjugated (bs-3274R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Rat muscle lysates probed with MKK3(Ser218) + MKK6(Ser207) Polyclonal Antibody, Unconjugated (bs-3274R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Phospho-MKK3(Ser218) + MKK6(Ser207)) Polyclonal Antibody, Unconjugated (bs-3274R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Phospho-MKK3(Ser218) + MKK6(Ser207)) Polyclonal Antibody, Unconjugated (bs-3274R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Phospho-MKK3(Ser218) + MKK6(Ser207)) Polyclonal Antibody, Unconjugated (bs-3274R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.