bs-3288R [Primary Antibody]
Phospho-MYPT1 (Thr853) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Phospho-MYPT1 (Thr853)

Specificity: This phosphorylation site is homologous to that of Thr852 in Mouse and Thr855 in Rat.

Modification Site: Thr853

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4659

Swiss Prot: O14974

Source: KLH conjugated synthetic phosphopeptide derived from human around the phosphorylation site of Thr853

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 113


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-Phospho-MYPT1 (Thr853) Polyclonal Antibody, Unconjugated (bs-3288R) at 1:200 followed by conjugation to the secondary antibody and DAB staining