Host: Rabbit
Target Protein: Mst1 Thr183 + Mst2 Thr180
Modification Site: Thr 183/Thr180
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6789, 6788
Swiss Prot: Q13043, Q13188
Source: KLH conjugated synthetic phosphopeptide derived from human Mst1 around the phosphorylation site of Thr183
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 19/37/56
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
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Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti Phospho-Mst1(Thr183)/Mst2(Thr180) Polyclonal Antibody, Unconjugated (bs-3294R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cell lysates probed with Phospho-Mst1(Thr183) + Mst2(Thr180) Polyclonal Antibody, Unconjugated (bs-3294R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cell lysates probed with Phospho-Mst1(Thr183) + Mst2(Thr180) Polyclonal Antibody, Unconjugated (bs-3294R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse spleen were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Mst1(Thr183)/Mst2(Thr180) Antibody(bs-3294R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).