bs-3330R-A647 [Conjugated Primary Antibody]
PERK(Thr980) Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PERK Thr980

Modification Site: Thr980

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 13666

Swiss Prot: Q9Z2B5

Source: KLH conjugated synthetic phosphopeptide derived from mouse PERK around the phosphorylation site of Thr980

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

Conjugation: ALEXA FLUOR® 647

Excitation/ Emission: 650nm/665nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 119


Cross Reactive Species: Human
Mouse
Rat
Dog
Pig

Predicted Cross Reactive Species: Cow
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Kucuksayan, Ertan, et al. "Neutral Sphingomyelinase Inhibition Decreases ER Stress-Mediated Apoptosis and Inducible Nitric Oxide Synthase in Retinal Pigment Epithelial Cells." Free Radical Biology and Medicine (2014).Read more>>
  • He, Yihuai, et al. "Sustained endoplasmic reticulum stress inhibits hepatocyte proliferation via downregulation of c-Met expression." Molecular and Cellular Biochemistry (2014): 1-8.Read more>>
  • Aslan, Mutay, et al. "Inhibition of Neutral Sphingomyelinase Decreases Elevated Levels of Inducible Nitric Oxide Synthase and Apoptotic Cell Death in Ocular Hypertensive Rats." Toxicology and Applied Pharmacology (2014).Read more>>
  • Xu, Demei, et al. "Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response and calcium release." Chemical Research in Toxicology (2015).Read more>>
  • Wang, Yu, et al. "Tanshinone II A Relieves Adriamycin-induced Myocardial Injury in Rat Model." International Journal of Chemistry 8.1 (2016): 40.Read more>>
  • Yu, H., et al. "Gypenoside Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting Cardiomyocytes Apoptosis via Inhibition of CHOP Pathway and Activation of PI3K/Akt Pathway In Vivo and In Vitro."Cellular Physiology and Biochemistry 39.1 (2016): 123-136.Read more>>
  • Chen, Yanan, et al. "Calcineurin β protects brain after injury by activating the unfolded protein response." Neurobiology of Disease (2016).Read more>>
  • Carmosino, Monica, et al. "The expression of Lamin A mutant R321X leads to endoplasmic reticulum stress with aberrant Ca2+ handling." Journal of Cellular and Molecular Medicine (2016).Read more>>
  • Yan, Jiting, et al. "Catalpol prevents alteration of cholesterol homeostasis in non-alcoholic fatty liver disease via attenuating endoplasmic reticulum stress and NOX4 over-expression." RSC Advances 7.2 (2017): 1161-1176.Read more>>
  • Mak, Shiu-Kwong, et al. "Tetramethylpyrazine suppresses angiotensin II-induced soluble epoxide hydrolase expression in coronary endothelium via anti-ER stress mechanism." Toxicology and Applied Pharmacology (2017).Read more>>
  • Gerbino, Andrea, et al. "Functional Characterization of a Novel Truncating Mutation in Lamin A/C Gene in a Family with a Severe Cardiomyopathy with Conduction Defects." Cellular Physiology and Biochemistry 44.4 (2017): 1559-1577.Read more>>
  • Santanam et al. Atg7 cooperates with Pten loss to drive prostate cancer tumor growth. (2016) Genes.De. 30:399-407Read more>>
  • Yan et al. Cytotoxicity of CdTe quantum dots in human umbilical vein endothelial cells: the involvement of cellular uptake and induction of pro-apoptotic endoplasmic reticulum stress. (2016) Int.J.Nanomedicine. 11:529-42Read more>>
  • Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167.Read more>>
  • Terzuoli,et al.Involvement of Bradykinin B2 Receptor in Pathological Vascularization in Oxygen-Induced Retinopathy in Mice and Rabbit Cornea.(2018) International Journal of Molecular Sciences. 19:.Read more>>
  • Wang,et al.Development of a SPR aptasensor containing oriented aptamer for direct capture and detection of tetracycline in multiple honey samples.(2018) Biosensors & Bioelectronics. 109:1-7.Read more>>
  • Liu.C. et al. Dexmedetomidine alleviates cerebral ischemia-reperfusion injury by inhibiting endoplasmic reticulum stress dependent apoptosis through the PERK-CHOPCaspase-11 pathway.(2018) Brain Research"Read more>>
  • Fang et al. Melatonin prevents senescence of canine adipose-derived mesenchymal stem cells through activating NRF2 and inhibiting ER stress. (2018) Aging.(Albany.NY). 10:2954-2972Read more>>
  • Kao et al. Opposite Regulation of CHOP and GRP78 and Synergistic Apoptosis Induction by Selenium Yeast and Fish Oil via AMPK Activation in Lung Adenocarcinoma Cells. (2018) Nutrients.ct 8;10(10). Read more>>
  • Ju Y et al. Protective effects of Astragaloside IV on endoplasmic reticulum stress-induced renal tubular epithelial cells apoptosis in type 2 diabetic nephropathy rats. (2019)Biomedicine & Pharmacotherapy. Jan;109:84-92. Read more>>
  • Cui J et al. Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress-and Ca2. Oxid Med Cell Longev. 2019 Jan 9;2019:2593742. Read more>>
  • Kim JE et al. TRPC6-Mediated ERK1/2 Activation Increases Dentate Granule Cell Resistance to Status Epilepticus Via Regulating Lon Protease-1 Expression and Mitochondrial Dynamics. Cells. 2019 Nov 1;8(11). pii: E1376.Read more>>
  • ingwei Yang et al. Polychlorinated Biphenyl Quinone Promotes Atherosclerosis through Lipid Accumulation and Endoplasmic Reticulum Stress via CD36. Chem Res Toxicol. 2020 Jun 15;33(6):1497-1507.Read more>>
  • Yan Luoet al. Dihydroartemisinin exposure impairs porcine ovarian granulosa cells by activating PERK-eIF2-ATF4 through endoplasmic reticulum stress. Toxicol Appl Pharmacol. 2020 Sep 15;403:115159.Read more>>
  • Wenfeng Gouet al. Ursolic acid derivative UA232 evokes apoptosis of lung cancer cells induced by endoplasmic reticulum stress. Pharm Biol. 2020 Dec;58(1):707-715.Read more>>
  • Yuting Wang. et al. Polybrominated diphenyl ethers quinone-induced intracellular protein oxidative damage triggers ubiquitin-proteasome and autophagy-lysosomal system activation in LO2 cells. Chemosphere. 2021 Jul;275:130034Read more>>
  • Xiaowei Sun. et al. Acupuncture protects against cerebral ischemiaCreperfusion injury via suppressing endoplasmic reticulum stress-mediated autophagy and apoptosis. Mol Med. 2020 Dec;26(1):1-14Read more>>
  • Xuejiao Zhou. et al. The novel ALK inhibitor ZX\29 induces apoptosis through inhibiting ALK and inducing ROS\mediated endoplasmic reticulum stress in Karpas299 cells. 2020 Nov 02Read more>>
  • Donghui Yang. et al. Melatonin alleviates LPS\induced endoplasmic reticulum stress and inflammation in spermatogonial stem cells. J Cell Physiol. 2021 May;236(5):3536-3551Read more>>
  • Ji-Eun Kim. et al. Epigallocatechin-3-Gallate and PEDF 335 Peptide, 67LR Activators, Attenuate Vasogenic Edema, and Astroglial Degeneration Following Status Epilepticus. Antioxidants-Basel. 2020 Sep;9(9):854Read more>>
  • Rui Zhang. et al. Apigetrin ameliorates streptozotocin-induced pancreatic -cell damages via attenuating endoplasmic reticulum stress. In Vitro Cell Dev-An. 2020 Sep;56(8):622-634Read more>>
  • Shaobin Yang. et al. Neuronostatin Promotion Soluble A1-42 Oligomers: Induced Dysfunctional Brain Glucose Metabolism in Mice. Neurochem Res. 2020 Oct;45(10):2474-2486Read more>>
  • Yu Wang. et al. Zinc offers splenic protection through suppressing PERK/IRE1-driven apoptosis pathway in common carp (Cyprinus carpio) under arsenic stress. Ecotox Environ Safe. 2021 Jan;208:111473Read more>>
  • ingbing Ma. et al. Dietary taurine supplementation ameliorates muscle loss in chronic heat stressed broilers via suppressing the perk signaling and reversing endoplasmic reticulum\stress\induced apoptosis. 2020 Oct 1Read more>>
  • Zhang Hao. et al. Protective effects of pterostilbene against hepatic damage, redox imbalance, mitochondrial dysfunction, and endoplasmic reticulum stress in weanling piglets. J Anim Sci. 2020 Oct;98(10)Read more>>
  • Yujie Zhong. et al. Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca 2+ homeostasis. 2021 Mar 02Read more>>
  • Ashley R. Helseth. et al. Cholinergic neurons constitutively engage the ISR for dopamine modulation and skill learning in mice. Science. 2021 Apr;372(6540)Read more>>
  • Wei Shi. et al. Identification of dihydrotanshinone I as an ERp57 inhibitor with anti-breast cancer properties via the UPR pathway. Biochem Pharmacol. 2021 Aug;190:114637Read more>>
  • Nadya Al-Yacoub. et al. Mutation in FBXO32 causes dilated cardiomyopathy through up-regulation of ER-stress mediated apoptosis. Commun Biol. 2021 Jul;4(1):1-12Read more>>
  • Ji-Eun Kim. et al. AMPA Receptor Antagonists Facilitate NEDD4-2-Mediated GRIA1 Ubiquitination by Regulating PP2B-ERK1/2-SGK1 Pathway in Chronic Epilepsy Rats. Biomedicines. 2021 Aug;9(8):1069Read more>>
  • Chang, Lei. et al. Attenuation of Activated eIF2 Signaling by ISRIB Treatment After Spinal Cord Injury Improves Locomotor Function. 2021 Oct 13Read more>>
  • Tao Tu. et al. Dietary -3 fatty acids reduced atrial fibrillation vulnerability via attenuating myocardial endoplasmic reticulum stress and inflammation in a canine model of atrial fibrillation. J Cardiol. 2021 OcRead more>>
  • Chen Meixue. et al. SMYD1 alleviates septic myocardial injury by inhibiting endoplasmic reticulum stress. Biosci Biotech Bioch. 2021 OcRead more>>
  • Jianlong Du. et al. FXR, a Key Regulator of Lipid Metabolism, Is Inhibited by ER Stress-Mediated Activation of JNK and p38 MAPK in Large Yellow Croakers (Larimichthys crocea) Fed High Fat Diets. Nutrients. 2021 Dec;13(12):4343Read more>>
  • Li Yi-Ming. et al. Procyanidin B2 Alleviates Palmitic Acid-Induced Injury in HepG2 Cells via Endoplasmic Reticulum Stress Pathway. Evid-Based Compl Alt. 2021;2021:8920757Read more>>
  • Fang Dong. et al. Trimethylamine N-oxide promotes hyperoxaluria-induced calcium oxalate deposition and kidney injury by activating autophagy. Free Radical Bio Med. 2021 NovRead more>>
  • I-Ta Lu. et al. (−)-Agelasidine A Induces Endoplasmic Reticulum Stress-Dependent Apoptosis in Human Hepatocellular Carcinoma. Mar Drugs. 2022 Feb;20(2):109Read more>>
  • Raines, Lydia N.. et al. PERK is a critical metabolic hub for immunosuppressive function in macrophages. Nat Immunol. 2022 Feb;23(3):431-445Read more>>
  • Fan Kexia. et al. Drp1-Mediated Mitochondrial Metabolic Dysfunction Inhibits the Tumor Growth of Pituitary Adenomas. Oxid Med Cell Longev. 2022;2022:5652586Read more>>
  • Wen-Chien Cheng. et al. Pendulone induces apoptosis via the ROS-mediated ER-stress pathway in human non-small cell lung cancer cells. Toxicol In Vitro. 2022 Jun;81:105346Read more>>
VALIDATION IMAGES