Host: Rabbit
Target Protein: PKC alpha/beta 2 Thr638/641
Modification Site: Thr638/641
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5578
Swiss Prot: P17252
Source: KLH conjugated synthetic phosphopeptide derived from human PKC alpha/beta II around the phosphorylation site of Thr638/641
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 77
Cross Reactive Species:
Mouse
Rat
Dog
Others
Predicted Cross Reactive Species:
Human
Cow
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Król, Magdalena, et al. "Macrophages Mediate a Switch between Canonical and Non-Canonical Wnt Pathways in Canine Mammary Tumors." PloS one 9.1 (2014): e83995.Read more>>
- Edens, Lisa J., and Daniel L. Levy. "cPKC regulates interphase nuclear size during Xenopus development." The Journal of Cell Biology 206.4 (2014): 473-483.Read more>>
- Edens et al. PKC-mediated phosphorylation of nuclear lamins at a single serine residue regulates interphase nuclear size in Xenopus and mammalian cells. (2017) Mol.Biol.Cel. 28:1389-1399Read more>>
- Rui Ding. et al. Endoplasmic reticulum stress and oxidative stress contribute to neuronal pyroptosis caused by cerebral venous sinus thrombosis in rats: Involvement of TXNIP/peroxynitrite-NLRP3 inflammasome activation. Neurochem Int. 2020 Dec;141:104856Read more>>
- Xin Jin. et al. GPRC6A is a key mediator of palmitic acid regulation of lipid synthesis in bovine mammary epithelial cells. CELL BIOL INT. 2022 AugRead more>>
- Jiawei Wang. et al. Validation of MAPK signalling pathway as a key role of paeoniflorin in the treatment of intrahepatic cholestasis of pregnancy based on network pharmacology and metabolomics. EUR J PHARMACOL. 2022 Nov;935:175331Read more>>
- Minghui Zhang. et al. Selenomethionine promotes ANXA2 phosphorylation for proliferation and protein synthesis of myoblasts and skeletal muscle growth. J NUTR BIOCHEM. 2023 Feb;:109277Read more>>
Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-PKC alpha/beta II (Thr638/641) Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-PKC alpha/beta II (Thr638/641) Polyclonal Antibody, Unconjugated (bs-3333R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n