bs-3334R [Primary Antibody]
PKM2 (Tyr105) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PKM2 Tyr105

Modification Site: Tyr105

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5315

Swiss Prot: P14618

Source: KLH conjugated synthetic phosphopeptide derived from human PKM2 around the phosphorylation site of Tyr105

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 58


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Novoyatleva T et al. Is PKM2 Phosphorylation Prerequisite for Oligomer Disassembly in Pulmonary Arterial Hypertension? Am J Respir Crit Care Med. 2019 Sep 5. Read more>>
  • Lifang Li. et al. Changes in the Expression of MIF and Other Key Enzymes of Energy Metabolism in the Myocardia of Broiler Chickens with Ascites Syndrome. ANIMALS. 2022 Jan;12(19):2488Read more>>
  • Zhang, Zhiyong. et al. Gut fungi enhances immunosuppressive function of myeloid-derived suppressor cells by activating PKM2-dependent glycolysis to promote colorectal tumorigenesis. EXP HEMATOL ONCOL. 2022 Dec;11(1):1-22Read more>>
  • Zhang, Zhiyong. et al. Gut fungi enhances immunosuppressive function of myeloid-derived suppressor cells by activating PKM2-dependent glycolysis to promote colorectal tumorigenesis. EXP HEMATOL ONCOL. 2022 Dec;11(1):1-22Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human liver carcinoma labeled with Anti-Phospho-PKM2 (Tyr105)Polyclonal Antibody, Unconjugated (bs-3334R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Protein: mouse liver lysates, 30ug; \nPrimary: Anti-Phospho-PKM2(Tyr105) (bs-3334R) at 1:300; \nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \nECL excitated the fluorescence; \nPredicted band size : 58kD\nObserved band size : 58kD\n


Formalin-fixed and paraffin embedded rat thyroid labeled with Rabbit Anti-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (bs-3334R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Formalin-fixed and paraffin embedded human endometrium tissue labeled with bs-3334R, Unconjugated (bs-3334R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n