bs-3345R [Primary Antibody]
Phospho-PLK1 (Ser137) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Phospho-PLK1 (Ser137)

Specificity: This phosphorylation site is homologous across the listed species.

Modification Site: Ser137

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5347

Swiss Prot: P53350

Source: KLH conjugated synthetic phosphopeptide derived from human PLK1 around the phosphorylation site of Ser137

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. Phosphorylates NEDD1. NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. Phosphorylates both ECT2 and RACGAP1, and thereby stimulates their interaction that is essential for the cleavage furrow formation. Promotes the central spindle recruitment of ECT2.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 68


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Phospho-PLK1 (Ser137) Polyclonal Antibody, Unconjugated (bs-3345R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse large intestine lysates; probed with PLK1 (Ser137) Polyclonal Antibody, unconjugated (bs-3345R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Human Jurkat cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human K562 cell lysates; Lane 5: Human U-2 OS cell lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human MDA-MB-231 cell lysates probed with Phospho-PLK1 (Ser137) Polyclonal Antibody, Unconjugated (bs-3345R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.