bs-33857R [Primary Antibody]
EIF4B Polyclonal Antibody
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Host: Rabbit

Target Protein: EIF4B

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1975

Swiss Prot: P23588

Source: Recombinant human EIF4B protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Size: 100uL

Concentration: 1mg/ml

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1:50-200)

Predicted Molecular Weight: 69


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Pancreas tissue lysates; Lane 2: Rat Pancreas tissue lysates; Lane 3: Human Hela cell lysates; Lane 4: Human A431 Cell lysates; Lane 5: Human 293T Cell lysates probed with EIF4B Polyclonal Antibody, Unconjugated (bs-33857R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.