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Host: Rabbit
Target Protein: Phospho-RPS6 (Ser235+Ser236)
Modification Site: Ser235+Ser236
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6194
Swiss Prot: P62753
Source: KLH conjugated synthetic phosphopeptide derived from human S6 Ribosomal Protein around the phosphorylation site of Ser235/236
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 27
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6 (Ser235+Ser236) Polyclonal Antibody, Unconjugated (bs-3388R) at 1:400 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Hela (H) cells were treated with or without Anisomycin (250ng/ml) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-RPS6 (Ser235+Ser236) polyclonal antibody, unconjugated (bs-3388R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6 (Ser235+Ser236) Polyclonal Antibody, Unconjugated (bs-3388R) at 1:400 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6 (Ser235+Ser236) Polyclonal Antibody, Unconjugated (bs-3388R) at 1:400 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6 (Ser235+Ser236) Polyclonal Antibody, Unconjugated (bs-3388R) at 1:400 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6 (Ser235+Ser236) Polyclonal Antibody, Unconjugated (bs-3388R) at 1:400 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6 (Ser235+Ser236) Polyclonal Antibody, Unconjugated (bs-3388R) at 1:400 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.