Host: Rabbit
Target Protein: SIRT1 Ser47
Modification Site: Ser47
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 23411
Swiss Prot: Q96EB6
Source: KLH conjugated synthetic phosphopeptide derived from human SirT1 around the phosphorylation site of Ser47
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 58/81
Cross Reactive Species:
Human
Mouse
Rat
Dog
Predicted Cross Reactive Species:
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Suzuki, Maiko, and John D. Bartlett. "Sirtuin1 and autophagy protect cells from fluoride-induced cell stress." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1842.2 (2014): 245-255.Read more>>
- Suzuki, Maiko, Cheryl Bandoski, and John D. Bartlett. "Fluoride induces oxidative damage and SIRT1/autophagy through ROS-mediated JNK signaling."Free Radical Biology and Medicine (2015).Read more>>
- Liu et al. Fenofibrate inhibits atrial metabolic remodelling in atrial fibrillation through PPAR-α/sirtuin 1/PGC-1α pathway. (2016) Br.J.Pharmacol. 173:1095-109Read more>>
- "Liu GZ et al. Aldosterone stimulation mediates cardiac metabolism remodeling via Sirt1/AMPK signaling in canine model.Naunyn Schmiedebergs Arch Pharmacol. 2019 Mar 9."Read more>>
- Jianbin Xue. et al. The Sirtuin\1 relied antioxidant and antiaging activity of 5,5\diferulic acid glucoside esters derived from corn bran by enzymatic method. J Food Biochem. 2020 Dec;44(12):e13519Read more>>
- Ivn Ballasch. et al. Ikzf1 as a novel regulator of microglial homeostasis in inflammation and neurodegeneration. BRAIN BEHAV IMMUN. 2023 Mar;109:144Read more>>
- Osamu Nakagawasai. et al. Peptides obtained by enzymatic decomposition of mackerel induce recovery from physical fatigue by enhancing the SIRT1-mediated antioxidant effect in the soleus muscle of mice. J PHARMACOL SCI. 2023 May;152:61Read more>>
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-SIRT1(Ser47) Polyclonal Antibody, Unconjugated(bs-3393R) 1:200 followed by conjugation to the secondary antibody and DAB staining\n
MCF-7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SIRT1(Ser47) Polyclonal Antibody(bs-3393R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SIRT1(Ser47) Polyclonal Antibody, Unconjugated (bs-3393R) at 1:200 overnight at 4°C, DAB staining.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SIRT1(Ser47) Polyclonal Antibody(bs-3393R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).