bs-3393R-A350 [Conjugated Primary Antibody]
SIRT1(Ser47) Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated
www.biossusa.com
[email protected]
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DATASHEET

Host: Rabbit

Target Protein: SIRT1 Ser47

Modification Site: Ser47

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23411

Swiss Prot: Q96EB6

Source: KLH conjugated synthetic phosphopeptide derived from human SirT1 around the phosphorylation site of Ser47

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

Conjugation: ALEXA FLUOR® 350

Excitation/ Emission: 346nm/442nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 58/81


Cross Reactive Species: Human
Mouse
Rat
Dog

Predicted Cross Reactive Species: Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Suzuki, Maiko, and John D. Bartlett. "Sirtuin1 and autophagy protect cells from fluoride-induced cell stress." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1842.2 (2014): 245-255.Read more>>
  • Suzuki, Maiko, Cheryl Bandoski, and John D. Bartlett. "Fluoride induces oxidative damage and SIRT1/autophagy through ROS-mediated JNK signaling."Free Radical Biology and Medicine (2015).Read more>>
  • Liu et al. Fenofibrate inhibits atrial metabolic remodelling in atrial fibrillation through PPAR-α/sirtuin 1/PGC-1α pathway. (2016) Br.J.Pharmacol. 173:1095-109Read more>>
  • "Liu GZ et al. Aldosterone stimulation mediates cardiac metabolism remodeling via Sirt1/AMPK signaling in canine model.Naunyn Schmiedebergs Arch Pharmacol. 2019 Mar 9."Read more>>
  • Jianbin Xue. et al. The Sirtuin\1 relied antioxidant and antiaging activity of 5,5\diferulic acid glucoside esters derived from corn bran by enzymatic method. J Food Biochem. 2020 Dec;44(12):e13519Read more>>
  • Ivn Ballasch. et al. Ikzf1 as a novel regulator of microglial homeostasis in inflammation and neurodegeneration. BRAIN BEHAV IMMUN. 2023 Mar;109:144Read more>>
  • Osamu Nakagawasai. et al. Peptides obtained by enzymatic decomposition of mackerel induce recovery from physical fatigue by enhancing the SIRT1-mediated antioxidant effect in the soleus muscle of mice. J PHARMACOL SCI. 2023 May;152:61Read more>>
VALIDATION IMAGES